Polynucleotides HPTTM -based compounds exhibit Scavenging Activity Against Reactive Oxygen Species

This study investigates the scavenger activity of Polynucleotides High Purification Technology (PN-HPTTM) and hyaluronic acid (HA) products and their combination (PN-HPTTM +HA) against oxidative stress induced by hydrogen peroxide (H₂O₂). Since oxidative stress is implicated in numerous pathological conditions, identifying effective antioxidants is crucial for therapeutic development. We employed a cell-free fluorometric assay using Calcein-AM to evaluate the dose- and time-dependent effects of these compounds in neutralizing reactive oxygen species (ROS). PN-HPTTM, HA, and PN-HPTTM +HA products were tested at various concentrations over multiple time points. Our results demonstrated that all tested treatments significantly lowered ROS levels compared to the untreated control. Notably, the PN-HPTTM -based compound exhibited robust scavenging activity, while the PN-HPTTM +HA based compound displayed the strongest and most consistent ROS-neutralizing effect across all concentrations and time points. This enhanced performance suggests a synergistic interaction between PN-HPTTM and HA, potentially due to complementary mechanisms of free radical scavenging and structural stabilization. These findings highlight the potential of PN-HPTTM and PN-HPTTM +HA based compounds as effective antioxidative agents, offering potential for therapeutic applications where oxidative stress is central, including wound healing and tissue regeneration.