Arsenite alters auxin transport and MAP kinase-mediated signaling in Arabidopsis thaliana L. root

Arsenite alters auxin transport and MAP kinase-mediated signaling in Arabidopsis thaliana L. root | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Arsenite alters auxin transport and MAP kinase-mediated signaling in Arabidopsis thaliana L. root Andreina Ocampo Santana, Salvador Barrera Ortiz, Luz de Maria Bretón-Deval, and 2 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-7077463/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract Root is the plant organ in direct contact with the soil, and the later contains many pollutants as heavy metals and metalloids. Arsenite (As [III]) is the most toxic form of inorganic arsenic (As), a metalloid affecting plant growth and development. Little is known about the signaling mechanism by which plants respond to the toxic effect of As [III]. In this report, a pharmacogenetic strategy was used to show that As [III] inhibits root growth in Arabidopsis thaliana L., arresting cell division and elongation, as well as driving cell damage in primary root. Since N-1-naphthylphthalamic acid (NPA; auxin transport inhibitor) enhances the inhibitory primary root effects of As [III], it was hypothesized that auxins are involved in the response. This hypothesis was verified using the pin2 mutant, which is hypersensitive to the As [III] effect and accumulates auxins in the RAM, as well as evaluating the expression levels of the IAA17 gene, which regulates auxin-induced gene expression and whose expression was inhibited by As [III]. Besides, a MAPK cascade was implicated in the signaling pathway activated by As [III], since GUS reporter gene expression of pMPK6::UidA and pMPK3::UidA was up-regulated in a dependent manner on As [III] concentration, although, most likely due to functional redundancy, mpk6-2 and mpk3-1 mutants behave like a wild-type during As [III] treatments. In summary, our results suggest that As [III] inhibits cell division and expansion, causing cell damage on primary root tip and blocking auxin transport, through a MAP kinase module. Arsenite Auxin transporter MAP kinase signaling MPK6 MPK3 PIN2 ABCB Full Text Supplementary Files SupplementaryMaterial.pdf Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. 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Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-7077463","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":489051921,"identity":"b9d6a022-8c4f-4071-881b-2cc345105146","order_by":0,"name":"Andreina Ocampo Santana","email":"","orcid":"","institution":"Instituto de Biotecnologia UNAM","correspondingAuthor":false,"prefix":"","firstName":"Andreina","middleName":"Ocampo","lastName":"Santana","suffix":""},{"id":489051922,"identity":"6874c267-398b-4dc7-9c7c-76586ab1554a","order_by":1,"name":"Salvador Barrera Ortiz","email":"","orcid":"","institution":"Instituto de Biotecnologia 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Arsenite (As [III]) is the most toxic form of inorganic arsenic (As), a metalloid affecting plant growth and development. Little is known about the signaling mechanism by which plants respond to the toxic effect of As [III]. In this report, a pharmacogenetic strategy was used to show that As [III] inhibits root growth in Arabidopsis thaliana L., arresting cell division and elongation, as well as driving cell damage in primary root. Since N-1-naphthylphthalamic acid (NPA; auxin transport inhibitor) enhances the inhibitory primary root effects of As [III], it was hypothesized that auxins are involved in the response. This hypothesis was verified using the pin2 mutant, which is hypersensitive to the As [III] effect and accumulates auxins in the RAM, as well as evaluating the expression levels of the IAA17 gene, which regulates auxin-induced gene expression and whose expression was inhibited by As [III]. Besides, a MAPK cascade was implicated in the signaling pathway activated by As [III], since GUS reporter gene expression of pMPK6::UidA and pMPK3::UidA was up-regulated in a dependent manner on As [III] concentration, although, most likely due to functional redundancy, mpk6-2 and mpk3-1 mutants behave like a wild-type during As [III] treatments. 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