SpaCir-VDJ: a broadly compatible circularization strategy for spatial immune repertoire profiling

Abstract Deciphering the spatial organization of immune clonal lineages is critical for understanding the evolution of adaptive immune responses. However, mainstream spatial transcriptomics platforms struggle to reconcile cost-effective short-read sequencing with the simultaneous and efficient capture of V(D)J sequences. To address this gap, we developed SpaCir-VDJ, an approach that utilizes a “multiplex PCR enrichment plus library circularization” strategy to integrate spatial barcodes and V(D)J CDR3 regions into a compact circular template optimized for standard short-read sequencing. This workflow is accompanied by an automated computational pipeline designed for circular-library data parsing, sequence reconstruction, spatial barcode deconvolution, and high-fidelity clonotype calling. In human lymphoid tissues, SpaCir-VDJ delineated germinal center (GC)-associated clonal organization and isotype-linked somatic hypermutation (SHM) patterns. Notably, lineage-based spatial analyses inferred inter-GC clonal redistribution events, suggesting that GCs in the analyzed samples may not be fully isolated reaction units. Functional perturbation provided orthogonal support for candidate SHM regulators identified by spatial analysis, with knockdown of MDM4, LYN, EAF2, and H2AFY each leading to reduced SHM. Finally, in gastric cancer, SpaCir-VDJ localized dominant BCR lineages within tertiary lymphoid structures and tumor-margin plasma cell–rich regions, with lineage patterns consistent with extension toward the tumor interior. We further observed a significant positive correlation between cumulative tumor mutations and spatial TCR diversity, consistent with a spatial association between local mutational burden and T-cell remodeling within the tumor microenvironment. Collectively, SpaCir-VDJ provides a scalable and cost-effective framework for interrogating the spatiotemporal dynamics of immune microenvironments. Competing Interest Statement The authors have declared no competing interest.