Improvement of Establishment of a Rat Model of Endometriosis and Observation of Its Microvessel Density (MVD)

Objective To evaluate the rat model of endometriosis (EMs),conducted by autotransplantation of its own endometrium between the superficial fascia and muscular layer of the abdominal wall. Methods 10 Female virgin rats in estrus stage were induced by estrogen injection prior to operation. During the laparotomy,the right uterus was taken and its endometrium was implanted into the left and right abdominal wall between superficial fascia and muscular layer. Two cysts were formed and they was taken out at 29 days after implantation,examined by histology and immunohistochemistry. Results The ectopic endometrium growing in the abdominal wall formed a cystic mass with a large cavity containing much mucus. It showed an endometrial structure similar to that of normal endometrium. Microvessel density (MVD) in the ectopic endometrium was higher in comparison with that of eutopic endometrium and normal endometrium. Conclusion These results suggested that the rat model established by this method is suitable for endometriosis research.