Immunoprofiling the peripheral blood of patients diagnosed with endometriosis 3686

Abstract Description Endometriosis is a debilitating condition which occurs due to ectopic growth of endometrial tissue. Inflammation is a normal feature of the menstrual cycle with dysregulated inflammation characteristic of endometriosis. These inflammatory changes are described predominantly within the uterus, but parallel changes systemically offer the opportunity to address knowledge gaps in our understanding of disease mechanisms to uncover novel treatments. We have optimised an immunoprofiling flow cytometry panel to incorporate measurements of cellular activation, exhaustion, chemokine receptor and mitochondria on a wide variety of immune cells subsets to analyse 568 data points in a small sample size. Peripheral blood was obtained from patients undergoing a diagnostic laparoscopy under approval of a Research Ethics Committee. Peripheral blood mononuclear cells were isolated and analysed using our 27-colour panel. Of the data points analysed, 88 were significant across a range of cell subsets. Most notable of these were changes of the CD8 T cell subsets, including increased expression of CD11c and CCR7 reminiscent of hyperinflammatory conditions. Combined with increased mitochondrial mass suggests that the peripheral CD8 T cells in endometriosis are over-activated and could offer a new therapeutic target. As we know immune cell metabolism impacts their fate and function, we are currently investigating the metabolic profile of isolated CD8 T cells combined with functional measurements. Funding Sources Supported by The Royal Society RGS\R2\242264; Saint David’s Medical Foundation SDMF24B Topic Categories Immune Mechanisms of Human Disease (HUM)