Human Papillomavirus Prevalence and Genotyping in Paraffin-Embedded Cervical Tumor Samples from Women in Kordofan State, Western Sudan

Abstract Background This study aimed to assess HPV prevalence and genotypic distribution in Sudanese cervical tumors, filling a global data gap and aiding screening and prevention efforts. Methods 121 FFPE blocks of cervical tumors with a mean age of 53 ± 14 years were collected. Despite 41 samples having degraded DNA, all underwent amplification with HPV-specific primers and human HLA-DQA1 gene as an internal control. DNA microarray chips was used to detected 41 HPV genotypes. Results A 121 cervical tumor specimens were analyzed using DNA microarray chip techniques to identify 41 HPV genotypes. The majority of age group (46.3%) falling into the ≥ 60 years age group. we found that the most predominant tumor is SCC (69.3%), with a significant age-tumor progression correlation (P = 0.001).