Grey Frequency-Based Methodology for Assessing HeLa Cell Damage

Abstract Cell biology techniques offer a solid foundation for evaluating and forecasting the danger of pollutants in the investigations of environmental toxicology. Studies on ecological toxicity, medication development, and illness diagnosis depend on evaluating cellular damage. The morphology of stimulated cells can alter the light scattering and reflection, and the brightness of microscopic images of the cells. This study demonstrated that stimulation-damaged and normal cells had distinct grey value distributions which led to the proposal of a novel theory to measure cellular damage by image brightness. Additionally, an evaluation technique based on grey frequency analysis can be created to determine the extent of cellular damage. This approach provides an effective and helpful tool for cellular damage visualization and quantitative evaluation in environmental toxicity assessment.