Biostimulation-phytoremediation of petroleum hydrocarbon contaminated soil: Response surface methodology (RSM)

Abstract In view of the analysis and remediation of petroleum hydrocarbon contaminated soil, the study on ecological technology of oilfield well site was carried out. The response surface was used to optimize the single-factor biological stimulation experiment of moisture content, the leavening agent content and the compound fertilizer content, and get the best experimental plan of biological stimulation. The artificial stimulation-plant combined remediation experiment was designed. Through screening 20 kinds of plant seeds suitable for growth, selected 5 plants with high tolerance to petroleum hydrocarbons. The artificial biostimulation-phytoremediation combined degradation experiment of petroleum hydrocarbons in contaminated soil was designed, and the degradation rate of petroleum hydrocarbons in soil under the optimal ecological remediation scheme was obtained. It was found that petroleum hydrocarbons degradation rate in soil after 70 days artificial stimulation experiment was 28.6%. Through the screening of 20 plants, peanut had the highest tolerance to petroleum hydrocarbons in soil, and the degradation rate in 70 days soil reached 31.1%. After 70 days of biostimulation-phytoremediation, the degradation rate in soil reached 38.9%.