Bioactive potential of polysaccharides produced by lactic acid bacteria

Bioactive potential of polysaccharides produced by lactic acid bacteria | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Article Bioactive potential of polysaccharides produced by lactic acid bacteria Radwa, E. Noureldein, Refaat, A. A, El-Dougdoug, K.A, Khadiga, A. Abou-Taleb1, and 1 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-5266666/v1 This work is licensed under a CC BY 4.0 License Status: Under Review Version 1 posted 12 You are reading this latest preprint version Abstract Lactic acid bacteria (LAB) have attracted considerable attention because of their ability to generate exopolysaccharides (EPS).Exopolysaccharides play a crucial role in human health, demonstrating notable antitumor, antioxidant, and antimicrobial properties. This study aimed to optimize the production of EPS by lactic acid bacteria LAB isolates and evaluate their bioactivities.LAB isolates were screened for EPS production under different culture conditions. Twenty isolates with promising bioactivities were selected and further studied. The best isolate was analyzed for total sugar content and its potential as an anticancer and antiviral agent. LAB cultures showed an increase in EPS production in modified MRS medium with an initial pH of 6.8 and 37 °C. The dry polymer weight (DPW) and viscosity of EPS varied between 1500 and 17500 mg/ll, with the K1 isolate exhibiting the highest DPW. EPS exhibited notable antioxidant and antibacterial properties, with CH3, P2 and P4 showing significant bioactivity. The CH3 isolate, identified as Lactobacillus brevis, producing EPS in sweet whey medium,exhibited potential anticancer effects against Caco-2 cells and antiviral activity against HAV. The dry polymer and viscosity did not show significant differences between isolates and conditions. In summary, EPS exhibits antioxidant, antimicrobial,anticancer, and antiviral properties, suggesting potential applications in the food industry and for human health. Biological sciences/Biochemistry Biological sciences/Biological techniques Biological sciences/Plant sciences Exopolysaccharides Lactic acid bacteria antioxidant activity antimicrobial activity anticancer antiviral Full Text Additional Declarations No competing interests reported. Cite Share Download PDF Status: Under Review Version 1 posted Editorial decision: Revision requested 09 Jul, 2025 Reviews received at journal 25 Jun, 2025 Reviewers agreed at journal 12 May, 2025 Reviews received at journal 11 Feb, 2025 Reviewers agreed at journal 16 Jan, 2025 Reviewers agreed at journal 18 Nov, 2024 Reviewers agreed at journal 05 Nov, 2024 Reviewers invited by journal 05 Nov, 2024 Editor assigned by journal 05 Nov, 2024 Editor invited by journal 05 Nov, 2024 Submission checks completed at journal 02 Nov, 2024 First submitted to journal 15 Oct, 2024 You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-5266666","acceptedTermsAndConditions":true,"allowDirectSubmit":false,"archivedVersions":[],"articleType":"Article","associatedPublications":[],"authors":[{"id":377146192,"identity":"18df4a78-616e-4727-92e2-d6750875094c","order_by":0,"name":"Radwa, E. 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This study aimed to optimize the production of EPS by lactic acid bacteria LAB isolates and evaluate their bioactivities.LAB isolates were screened for EPS production under different culture conditions. Twenty isolates with promising bioactivities were selected and further studied. The best isolate was analyzed for total sugar content and its potential as an anticancer and antiviral agent. LAB cultures showed an increase in EPS production in modified MRS medium with an initial pH of 6.8 and 37 °C. The dry polymer weight (DPW) and viscosity of EPS varied between 1500 and 17500 mg/ll, with the K1 isolate exhibiting the highest DPW. EPS exhibited notable antioxidant and antibacterial properties, with CH3, P2 and P4 showing significant bioactivity. The CH3 isolate, identified as Lactobacillus brevis, producing EPS in sweet whey medium,exhibited potential anticancer effects against Caco-2 cells and antiviral activity against HAV. 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