Synthesis and Characterization of Ag Nanoparticles as Cholesterol Biosensor

In the present research work, the Ag NPs were synthesized by chemical reduction method using AgNO 3 as a reducing agent and NaBH 4 as stabilizing agent and reducing agent as well. The structural properties and crystallite size of the prepared sample were investigated via X-ray diffraction (XRD) spectroscopy. XRD revealed the cubic structure. Fourier transform infrared spectroscopy (FTIR) was used to investigate the chemical bond vibrations of the different groups present in Ag NPs. UV-Vis spectroscopy was done to observe the abortion spectrum of Ag NPs. The surface morphology was examined via scanning electron microscopy (SEM) and transmission electron microscopy (TEM). Afterwards, synthesized AgNPs have been used for the fabrication of cholesterol biosensor using three electrode system. The glassy carbon electrode (GCE) was used as working electrode. Finally the electrochemical measurements have been performed for the different concentration of cholesterol. The linearity response was observed between the potential difference and concentration of cholesterol, which shows that our material is effective for the fabrication of cholesterol biosensor.