Evaluating kinetic factors of peroxidase-like MnFe2O4 nanozymes

In this study, the kinetic factors of the peroxidase-like MnFe 2 O 4 nanozymes, including affinity factor, K m , and maximal velocity, V max , were calculated via excremental investigation. The OPD-mediated enzyme assay was utilized for determining the reaction rates and consequently constructing the saturation curves. Michaelis–Menten kinetic model was used as the standard kinetic model of enzymatic reactions while the linear plot of Lineweaver–Burk was constructed to provide reliable value for each factor. The results indicated a V max of 0.12 µM/sec for the peroxidase-like MnFe 2 O 4 nanozymes. Besides, the substrate affinity factor, K m , was estimated at about 27.7 mM for the peroxidase-like MnFe 2 O 4 nanozymes, exhibiting the high affinity of MnFe 2 O 4 nanozymes toward OPD.