In vitro peptidoglycan degradation assay using FITC labelled sacculi

2026 · doi:10.1099/acmi.0.001178.v1
preprint OA: closed
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Abstract

We describe a method using fluorescein-5-Isothiocyanate (FITC) labeled peptidoglycan sacculi to characterize peptidoglycan hydrolases in vitro. In this protocol soluble hydrolytic products released by peptidoglycan hydrolases are separated from the insoluble FITC-sacculi, quantified and set in relation to the overall available substrate. This enables the study of hydrolase activity in end-point or time- course experiments.
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Full text loading... Abstract We describe a method using fluorescein-5-Isothiocyanate (FITC) labeled peptidoglycan sacculi to characterize peptidoglycan hydrolases in vitro. In this protocol soluble hydrolytic products released by peptidoglycan hydrolases are separated from the insoluble FITC-sacculi, quantified and set in relation to the overall available substrate. This enables the study of hydrolase activity in end-point or time- course experiments. - Received: - Version Posted: Funding - URKI (Award MR/V027204/1) - Principal Award Recipient: Manuel Banzhaf

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last seen: 2026-05-20T01:45:00.602351+00:00