Ultra-fast micro-CT of an unrestrained live insect

Micro-CT has revolutionized functional morphology by enabling volumetric reconstruction of biological specimens at micrometre scales, but its accuracy is compromised by fixation artefacts. State-of-the-art in vivo imaging avoids this, but still requires subjects to be tethered, anaesthetized, or stained. Here we use ultra-fast synchrotron-based micro-CT to produce the first 3D scan of an unrestrained living organism at micrometre resolution, demonstrating the potential of this method in physiology, behaviour, and biomechanics.