Diagnostics and Therapy for Malignant (Degenerate) Colon Endometriosis - Three Case Reports

! Endometriosis is an oestrogen-dependent, prolif- erative disease that affects ca. 4 to 8 % of all wom- en in child-bearing age. It is in principle a benign disease; however, it does exhibit the properties of progression, neoangiogenesis, invasion and organ destruction that also characterise malignant pro- cesses. Sampson described for the first time the malignant degeneration of peritoneal endome- triosis [1] and ovarian endometriosis [2], and also formulated the criteria that are still valid today to prove histologically the malignant degeneration of endometriosis: 1. both carcinomatous and benign endometrial tissue must be detectable in the same organ, 2. cancer structures and benign endometrial tis- sue must be histologically correlated like ad- enocarcinoma of the uterus is to the endome- trium,

! Malignant degeneration of colon endometriosis is a very rare event. We report here on three cases. A 48-year-old woman with a 10-year history of en- dometriosis was treated for a rectal adenocarci- noma, a 61-year-old G1P1, who was operated at the age of 40 years for ovarian endometriosis and again at the age of 53 years for an endometriosis- associated endometroid ovarian carcinoma, pre- sented for therapy for a lymph node recurrence of the ovarian cancer and, secondly, due to a ma- lignantly degenerated rectum-sigmoid colon en- dometriosis; furthermore a 54-year old woman with a 21-year history of endometriosis was oper- ated for malignant colon endometriosis. The tu- mour occurred during an adjuvant anti-oestrogen treatment with an aromatase inhibitor following surgical and radiotherapy for breast cancer. In all cases a radical cancer operation was followed by adjuvant chemotherapy and in one case with an additional radiotherapy. In the follow-up periods of 18 months, 2 and 5 years, respectively, all women remained free of recurrences. Although this is not a randomised controlled study due to the rare occurrence of such cases, a radical opera- tion followed by individualised adjuvant therapy appears to be the treatment of choice. Zusammenfassung ! Die maligne Entartung einer Darmendometriose ist sehr selten. Wir berichten über 3 Fälle. Eine 48-jährige Patientin mit 10-jähriger Endometrio- seanamnese wurde wegen eines Adenokarzinom des Rektums behandelt; eine 61-jährige G1P1, die erstmals im Alter von 40 Jahren wegen Ova- rialendometriose und dann im Alter von 53 Jah- ren wegen eines Endometriose-assoziierten en- dometrioiden Ovrialkarzinoms operiert wurde, kam wegen Lymphknotenrezidiv des Ovarialkar- zinoms und zweitens wegen einer maligne ent- arteten Rektum-Sigma-Endometriose zur Thera- pie; ferner wurde eine 54-jährige Patientin, die seit 21 Jahren an Endometriose litt, wegen malig- ner Darmendometriose operiert. Der Tumor war während einer adjuvanten Antiöstrogenbehand- lung mit einem Aromatasehemmer nach operier- tem und bestrahltem Mammakarzinom entstan- den. In allen Fällen erfolgte eine radikale Karzi- nomoperation gefolgt von adjuvanter Chemo- therapie und in einem Fall zusätzlich adjuvanter Radiatio. Im Nachuntersuchungszeitraum von 18 Monaten, 2 bzw. 5 Jahren sind alle Frauen bis- her rezidivfrei. Obwohl es keine randomisierten, kontrollierten Studien wegen der Seltenheit der Fälle gibt, scheint die radikale Operation gefolgt von einer individualisierten adjuvanten Therapie die Behandlung der Wahl. Diagnostics and Therapy for Malignant (Degenerate) Colon Endometriosis – Three Case Reports Zur Diagnostik und Therapie maligne entarteter Darmendometriose – 3 Fallberichte Authors R. Schutz 1, J. Woziwodzki 2, K.-W. Schweppe 1 Affiliations 1 Endometriosezentrum Ammerland, Frauenklinik, Ammerland-Klinik GmbH, Akademisches Lehrkrankenhaus der Medizini- schen Hochschule Hannover, Westerstede 2 Pathologisches Institut Aurich/Westerstede, Westerstede Key words l" bowel endometriosis l" malignant degeneration l" endometriosis‑associated carcinoma Schlüsselwörter l" Darmendometriose l" maligne Entartung l" Endometriose‑assoziiertes Karzinom received 6. 8. 2015 revised 9. 11. 2015 accepted 17. 11. 2015

DOI http://dx.doi.org/ 10.1055/s-0041-109769 Geburtsh Frauenheilk 2016; 76: 417–422 © Georg Thieme Verlag KG Stuttgart · New York · ISSN 0016‑5751 Correspondence Prof. Karl-Werner Schweppe Endometriosezentrum Ammerland Frauenklinik Ammerland-Klinik GmbH Akademisches Lehrkrankenhaus der Medizinischen Hochschule Hannover Lange Straße 38 26655 Westerstede [email protected] 417 Schutz R et al. Diagnostics and Therapy … Geburtsh Frauenheilk 2016; 76: 417 –422 Case Report Deutsche Version unter: www.thieme-connect.de/ ejournals/gebfra 3. the adenocarcinoma has genuinely arisen in the organ. Corner and co-workers [3] additionally required histological evi- dence for a gradual transition from benign to malignant struc- tures. Beside the genital manifestations, an extragenital endometrial at- tack is found in up to 30 % of the cases. These are above all colon and bladder endometrioses whereas other locations such as lungs, lymph nodes, skin etc. are very rare. With a risk of up to ca. 1 % (see [4]) the malignant degeneration of an extragenital en- dometriosis is a rare event. We report on 3 cases of malignant rectum-sigmoid colon endo- metriosis and discuss the clinical problems on the basis of litera- ture reports. Case Reports ! Case 1 A 48-year-old woman with an external diagnosis of a sigmoid co- lon carcinoma was referred to the surgical department for opera- tive management. On account of dysmenorrhoea, hypomenor- rhoea and uterus myomatosus, surgery involved myoma enuclea- tion, adhesiolysis, and right-sided adnexectomy since an ovarian endometriosis was diagnosed intraoperatively. A subsequent en- docrine therapy was not initiated. In 2007 her general practitioner prescribed a gastroenterological examination to clarify perimenstrual pain and stool irregularities with slime and blood mixed in the stool. Colonoscopy revealed at 20 cm from the anus onwards polypoid protruding mucous membranes with a slight restriction of the lumen, tissue samples taken here exhibited a regularly formed colon mucosa. An MRI scan of the pelvis revealed a left-sided cystic ovarian lesion to- gether with small sigmoid lesions, and an intramural contrast be- haviour suggestive for endometriosis. In 2010 a laparoscopic supracervical hysterectomy with left- sided adnexectomy and extensive adhesiolysis was performed. No information is available about the suspicion of endometriosis from the previously performed MRI scan. Histology revealed multiple leiomyomas, internal adenomyosis, regressing endome- triosis cysts and a haemorrhagic corpus luteum cyst on the left ovary. The patient was 44 years old at this time and received no further gynaecological therapy and no hormone substitution after surgi- cal castration. With adipositas per magna (BMI 35) there were hardly any complaints about menopausal symptoms. In June 2013 diagnostic work-up initiated by her general practi- tioner due to bloody diarrhoea led to the histological diagnosis of sigmoid colon cancer. Sonographic and radiological staging ex- aminations did not provide any indications for metastasis. The tumour markers CEA and CA 19-9 were elevated at 27 ng/mL and 600 U/mL, respectively. On the gynaecological examination a cystic, partly echo-poor, partly echo-rich, poorly delineated 65 × 37 × 40 mm resistance with internal structures was found behind and above the cervical stump, giving rise to the sono- graphic and palpatory suspicion of recurring endometriosis of the posterior compartment including the rectovaginal septum. An interdisciplinary re-re-laparotomy revealed after extensive adhesiolysis the tumour at the level of the retrosigmoid junction as well as further parietal tumour elements attached to the rec- tum. Since the frozen section analysis showed evidence for an ad- enocarcinoma, a rectum resection with end-to-end anastomosis was performed with the tumour being removed in toto together with the in conglomerate clogged cervix uteri. This was followed by lymphadenectomy. In the final histology, which was con- firmed by an independent pathologist, pronounced endometrio- sis was found in the intestinal wall reaching through to the mus- cularis propria and submucosa. 18 of 46 regional lymph nodes had been attached by metastases, oral and aboral anastomosis rings were tumour-free, as was the cervix uteri. On consideration of the immunohistochemical characteristics (l " Fig. 1 and Table 1) of the adenocarcinoma, it was classified as an endometrial carcinoma on the basis of colorectal endometrio- sis in the region of the left and right adnexa in the condition after ovarian endometriosis (pT2 L1 V0 pNx pM1 [LYM] R0 G2). There were no complications in the postoperative course; the in- dication for adjuvant chemotherapy with carboplatin and Taxol was given. The patient received 6 cycles in the appropriate doses and intervals. In the follow-up period of to date 18 months there has been no evidence for a recurrence. Case 2 A 61-year-old woman was admitted to hospital in 2008 due to persisting lower abdominal and back pain with the suspicion of a lower abdominal tumour. In 1987 the then 40-year-old patient underwent adhesiolysis, extirpation and management of endo- metriosis because of situation of the adhesions, lower abdominal pain and a 7-cm long endometrioma in the vicinity of the left ovary; subsequent endocrine therapy was not initiated. In 1993 hysterectomy, left-sided adnexectomy as well as extensive adhe- siolysis were necessary due to recurrent endometriosis of the left adnexa with therapy-refractory lower abdominal pain as well as hyper- and dysmenorrhoea. Histology confirmed the deep infil- trating ovarian endometriosis together with adenomyosis uteri interna. Table 1 Immunohistochemical findings in the 3 cases. IHC Tissue CK7 CK20 ER PR PAX8 WT1 CD10 P53 Case 1 benign endometriosis malignant endometriosis + + – – + + + – + + – – + + – – Case 2 benign endometriosis malignant endometriosis + + – (+) + (+) + – + + – – + + – – Case 3 benign endometriosis borderline components malignant endometriosis + + + – – (+) + + – + – – + + + – + + + + – – – + + = positive, (+) = weakly positive, – = negative, IHC = immunohistochemistry, ER = oestrogen receptor, PR = progesterone receptor, CK7 = immunohistochemical marker for epi- thelial tumours, CK20 = immunohistochemical marker for intestinal tumours, PAX-8 = immunohistochemical marker for Müller ʼs epithelium, WT1 = immunohistochemial marker for serous tumours, CD10 = immunohistochemical marker for endometrial stroma, P53 = immunohistochemical marker for high-grade endometrial cancer 418 Schutz R et al. Diagnostics and Therapy … Geburtsh Frauenheilk 2016; 76: 417 –422 GebFra Science During 1997 the patient complained of moderate menopausal symptoms which, however, did not require hormone substitution therapy. In 2000 the patient underwent a renewed re-laparoto- my due to right-sided lower abdominal pain and a sonograph- ically as well as palpably unclear, right-sided, adnexa process of 10 × 8 × 7 cm in size. Histology revealed a moderately differenti- ated endometroid adenocarcinoma (pT1c G1 –2), which was op- erated appropriately for its stage. The staging procedure did not reveal any lymph node metastases, or any pulmonary, bone or liver metastases. CA 12-5 with 13 U/mL was in the normal range; the tumour cells were hormone receptor positive (PR 90 %, ER 20 %). Adjuvant gestagen therapy with megestrol acetate 40 mg daily was indicated. After about 5 years the patient terminated this therapy due to unacceptable side effects (weight gain, de- pressive moods). In 2008 the patient complained again about increasing lower ab- dominal pain. During the diagnostic work-up a large cherry- sized, poorly moveable resistance was found just above the stub of the vagina, which was not well delineated in the cranial direc- tion, on sonography a 19 × 18 × 25 mm cystic, partly solid tumour was visualised that could not exactly be delineated from the pos- terior bladder wall and the anterior wall of the rectum. A re-re-relaparatomy was performed due to the suspicion of a re- currence. After adhesiolysis palpable tumour formations were detected pararectally deep behind the stub of the vagina and in the region of the sigmoid colon. In the mesosigmoid enlarged lymph nodes were conspicuous, an intraoperative frozen section analysis revealed metastatic infiltration by an adenoid, partly pa- pillary structured tumour that is in accord with a primary meta- static endometrial carcinoma. A deep anterior rectum resection with lymphadenectomy was performed. The final histological analysis demonstrated two types of tumour formations: 1. a poorly differentiated ER-negative, PR-negative adenocarcino- ma in the vicinity of the regional lymph nodes, the mesocolon and mesorectum as well as paraaortically, 2. cystic dilated endometriosis structures with atypically trans- formed epithelial formations in the form of a papillary-serous carcinoma in situ with tumour propagation in the region of the intramural neural plexus of the intestinal wall and invasive tu- mour elements in the region of the intestinal wall endometrio- sis. In addition, focal dilated endometriosis cysts without atyp- ical cell formations. The resection margins were free of tumour. Eight of the total of 14 removed lymph nodes had been attacked by metastases. In conclusion, the findings were classified on the one hand as en- dometriosis of the rectum-sigmoid colon with progressive dys- plasia, carcinoma in situ and perineural invasion and, on the oth- er hand, as lymph node recurrence of the endometrial ovarian Fig. 1 a to d Cross-section through the tumorous thickened intestinal wall with a slice of the lumen (case 1). One can see the normal colon mucosa (on the right of the picture) and the cancerous pockets in the intestinal wall. a Haematoxylin and eosin staining, magnification 10 ×. b CK7 immunohis- tochemical staining with positive tumour cells and negative intestinal muco- sa that excludes a primary adenocarcinoma of the intestine. c Staining of the oestrogen receptors shows a weakly positive reaction of the malignant cells. d CK20 immunohistochemical staining shows negative tumour cells and positive reaction of the intestinal mucosa, in accord with the CK7 findings. 419 Schutz R et al. Diagnostics and Therapy … Geburtsh Frauenheilk 2016; 76: 417 –422 Case Report carcinoma treated surgical and with adjuvant therapy 8 years previously. The patient received an adjuvant chemotherapy with carboplatin AUC5 and Taxol (175 mg/m 2) in the appropriate doses and intervals. All follow-up examinations of the abdomen were unremarkable. A second recurrent disease had not occurred at five years after surgery. It should be mentioned that two years ago the patient underwent breast-conserving surgery and received adjuvant therapy for a poorly differentiated invasive ductal breast cancer (pT1c pTIS L0 V0 pN0 [SN 0 –1] R0). The patient has also not suffered from a re- currence of the breast disease. Case 3 A 54-year-old woman was admitted to hospital in 2009 because of recurring abdominal pain, constipation, loss of appetite and loss of energy with an unclarified tumour in the lesser pelvis. 21 years before, the then 33-year-old woman underwent a hysterec- tomy and resection of a deeply infiltrating parametric and retro- vaginal septum endometriosis by laparotomy on account of re- current, therapy-resistant hypermenorrhoea and dysmenor- rhoea. A postoperative endocrine therapy was not indicated. In 1993 a continuous gestagen therapy with medrogestone 5 mg daily was started because of a suspected recurrent endometriosis (cystic ovarian endometriosis). This was stopped after 14 months when the patient was diagnosed with a left-sided invasive ductal breast cancer (pT2 pN0 [SN] M0 G3, ER 40 %, PR 30 % Her2/neu- positive). In 1995 a breast-conserving operation with radiothera- py and adjuvant chemotherapy was carried out (4 cycles of EC scheme followed by an antioestrogen therapy with anastrozole 1 mg/d). Furthermore an adjuvant bilateral laparoscopic adnex- ectomy was performed (histology of the ovaries did not show any evidence of endometriosis, merely functional cysts and se- rous membrane inclusion cysts). On clinical examination and vaginal sonography, a good table- tennis ball-sized, firm elastic, immobile tumour was conspicuous above and dorsal from the vaginal stub, the rectal mucous mem- Fig. 2 a and b Intestinal wall with benign endometriosis (case 3). Hyperplas- tic thickened intestinal wall with cystic dilated endometriosis glands ( a), filled with secretions with flattened, inactive epithelium. In addition, islands with proliferating endometriosis surrounded by fibrosis and muscle cells (b). stain- ing HE; magn. a = 10× and b = 100×. Fig. 3 a and b Varying differentiation of the malignancy. The dedifferentia- tion spectrum of the tumour in case 3, classified as G2, ranges from border- line parts ( a) with epithelial high-grade atypical cell conglomerates without detectable invasion through to little differentiated carcinoma cells in the lymph node metastases ( b). 420 Schutz R et al. Diagnostics and Therapy … Geburtsh Frauenheilk 2016; 76: 417 –422 GebFra Science branes could be moved. A colonoscopy performed 7 months ear- lier because of constipation and lower abdominal pain did not re- veal any abnormal findings. An MRI scan demonstrated a 3-cm, solid tumorous structure above the vaginal stub that could not be exactly delineated from the rectal wall. With the suspicion of recurrent endometriosis a re-laparotomy was performed and, after difficult adhesiolysis of the frozen pel- vis, a mandarin-sized tumour reaching from above and dorsolat- eral of the vaginal stub to the paraproctium was detected. The frozen section analysis confirmed the clinical suspicion of malig- nancy so that after total mesorectal mobilisation a deep anterior rectum resection with end-to-end anastomosis was performed. The histological analysis revealed a moderately differentiated en- dometrial adenocarcinoma (32 mm in size) on the bed of a previ- ously existing endometriosis (l " Fig. 2) in the vicinity of the rectal wall with infiltration into all layers of the wall through to the submucosa. In the vicinity of one tumour part changes were seen that corresponded to a borderline tumour. Of 26 regional lymph nodes only one had been attacked ( l " Fig. 3). The fibrolipomatous pelvic connective tissue (residual parametria) was tumour-free, as was also the resected vaginal stub. The patient received an ad- juvant therapy comprised of 6 cycles of cisplatin/doxorubicin and in sequence radiotherapy of the lesser pelvis and pelvic lymph drainage pathways until January 2010. Tumour follow-up find- ings including imaging procedures (sonography and MRI of the lesser pelvis) have remained unremarkable up to date.

! Malignant degeneration of endometriosis is a rare event and ma- lignant degeneration of intestinal endometriosis is extremely rare. During the 5-year period reported here (2008 –2012) we have operated on 3416 patients for endometriosis; 263 of them for rectum-sigmoid colon endometriosis (7.7 %). We observed 13 endometriosis-associated malignancies (0.38 % of all operated endometriosis cases); among them were the 3 cases described here. This corresponds to an incidence for malignant degenera- tion of extragenital cases of 23 % of all endometriosis-associated malignancies or, respectively, 0.88 per thousand of all endome- triosis cases and 1.14 % of all intestinal endometrioses. Thus, our figures are lower than those given in the literature over the past few years where a risk of 2.5 % for the malignant degeneration of ovarian endometriosis was calculated (6 × higher than in our col- lective) [5]. These differences can be explained by considering that the in part low case numbers, the nature of the publishing facility (gynaecology, surgery, pathology) and specialisation of the hospital all have a strong influence on the investigated collec- tives. An interdisciplinary, multicentre trial is needed to provide valid incidence data and clinically relevant risk estimations; such a trial is currently being carried out by Ulrich and co-workers [6]. According to a recent literature review [7] 80 % of all extragonadal endometriosis-associated malignancies have their origins in the rectum-sigmoid colon and on histology two thirds of the cases prove to be adenocarcinomas, 10 % each endometroid stromal sarcomas and adenosarcomas as well as malignant Müller ʼs mixed tumours in 5 % of the cases. Thus, our three cases corre- spond to the most frequent location and the most frequent histo- logical type. The differential diagnosis between endometroid adenocarcino- ma and primary adenocarcinoma of the colon is difficult, not only for the clinician but also for the pathologist. The symptomatics are identical, but clinically the lack of attack on the intestinal mu- cous membranes points to an endometroid process, ultimately, however, immunohistochemical examinations are necessary to make an exact diagnosis. A primary colon carcinoma is CK-20 positive and CK-7 negative whereas, in contrast, an endometroid carcinoma is CK-7 positive and CK-20 negative (for details of the cases, see l " Tab. 1). However, since individual tumour parts can exhibit different receptor expressions and immunohistochemical reactions (case 1), the differential diagnosis can be problematic. This is also apparent for case 3 where the negative response for oestrogen receptors can be considered as a sign of dedifferentia- tion, whereas the CD 10 negative with positive P53 result sug- gests for these differently differentiated carcinomas there are al- so areas that correspond to a highly differentiated endometroid carcinoma. Furthermore, it is a matter of discussion if the TNM classification is meaningful for extragenital malignant endometriosis and whether it should be classified as an ovarian or an intestinal can- cer. Thus, for example, this question can be posed in case 3 in which a hysterectomy had been performed in 1988, a bilateral adnexectomy in 1995 and in whom in the absence of internal genital organs a malignant colon endometriosis was operated in 2009. As a colon carcinoma the classification pT3L0V0pN1 (1/26) pMx R0 G2 would have been correct because the malignancy had originated from tissue of the genital organs while pT3 pNx pM1 (LYM + intestine) G2 would have been logical. Similarly in case 1, in whom in 2003 a myoma enucleation with right-sided adnexec- tomy and in 2010 a supracervical hysterectomy with left-sided adnexectomy were performed, both because of endometriosis and myomas. In 2013 a sigmoid colon resection and lymphade- nectomy with resection of the cervix uteri were carried out. For an intestinal carcinoma the classification pT2L1V0pN2 (18/46) pMx R0 G2 would be correct whereas for an ovarian carcinoma pT2L1V0pNxpM1 (LYM) R0 G2 would be correct. For the surgical procedure the assignment as intestinal carcinoma is helpful whereas for the adjuvant therapy the phenotype of the carcino- ma is rather more relevant. Accordingly, the locoregional lymph nodes of the colon are removed surgically, the adjuvant chemo- therapy with carboplatin and Taxol then takes the metastatic (pM1 LYM) endometroid carcinoma into consideration. Since no tumour formula is appropriate for the situation of extragonadal malignant endometriosis, it is recommended to disregard the or- gan classification according to the TNM system and to descrip- tively report the pathological findings phenotype, tumour size and resection margins, lymph node attack and metastasis. At the time of diagnosis the average age was 55.4 years with a standard deviation of 12.8 years, whereby at this time the youn- gest patient was 33 years old and the oldest 80 years [7]. Our cases were also peri- or, respectively, post-menopausal women and Ulrich and co-workers [6] pointed out that in cases of recur- rence of endometriosis symptoms in peri- and post-menopausal patients the clinician should always consider the possibility of a malignant degeneration of the already known and documented endometriosis. In many of the previously published cases the patients had re- ceived long-term oestrogen monotherapies, but endometriosis- associated carcinomas have also been described under Taxol or in one case, respectively, under gestagen therapy [8]. We ob- served carcinomas without any influence of endocrine therapy (case 1) or, respectively, after terminated gestagen therapy (case 2) and during an antioestrogen therapy with an aromatase inhib- 421 Schutz R et al. Diagnostics and Therapy … Geburtsh Frauenheilk 2016; 76: 417 –422 Case Report itor (case 3). The latter situation had not been reported previ- ously. Individual case reports and small series do not allow for any clear recommendations about endocrine therapy for postmenopausal symptoms in patients with endometriosis; however, an indicated oestrogen administration should always be combined with a ges- tagen [9]. The pathophysiological background for this recom- mendation is that oestrogens not only stimulate the proliferation of oestrogen receptor-positive cells but also influence the prosta- glandin metabolism by means of an increased cyclooxygenase ac- tivity. This favours a resistance to apoptosis and in relation with the hyperoestrogenism (favoured locally by aromatase activity) leads to a higher risk for malignant transformation. Various other molecular biological mechanisms for endometriosis-associated malignancies of the ovary have been discussed. Thus an Fe ++- overload in the endometriosis lesion can lead to protein changes and DNA damage through an elevation of oxygen radicals [10] and inactivation of the PTEN tumour suppressor gene as the first step for a malignant transformation [11]. It has not yet been ex- amined if this mechanism is also applicable for the malignant de- generation of extragonadal endometriosis. The clinical picture of extragonadal endometriosis frequently re- flects its localisation [12]. Vaginal bleeding following a hysterec- tomy, rectal bleeding from the posterior compartment in a pa- tient with a history of unspecific lower abdominal pain have also been reported along with stool irregularities, constipation and intestinal cramps. Thus, in the diagnostic work-up an interdisci- plinary procedure has proved valuable since colonoscopy is often unremarkable, as pointed out by Yantiss and co-workers [4] in their series of 17 cases. Rectovaginal palpation, sonography and radiological imaging procedures point in the right direction but only histology is decisive. Surgical treatment follows the principles of cancer surgery in the lesser pelvis with the target of R0 resection and regional lym- phadenectomy. Chemotherapy with platinum-containing sub- stances combined with taxanes is recommended as an adjuvant therapy [13], which was also performed in our case 1 whereas in case 3 a combination of platinum with anthracycline was admin- istered. On the other hand, it has been suggested that patients with, above all, extragonadal endometriosis-associated malignancies that are limited in extent to the lower pelvis, will benefit more from adjuvant pelvic radiotherapy [8]. Furthermore, a high-dose gestagen therapy may possibly have a similar effect on gestagen receptor-positive malignancies as is known from the therapy for endometroid cancer. This was the reason in our case 2 for the ad- juvant high-dose gestagen therapy for the endometroid ovarian cancer stage I c. There are also discrepancies in the literature with regard to the prognosis. Some authors found no differences between endome- triosis-associated and non-endometriosis-associated malignan- cies [14, 15] while others found a better prognosis of EAM [8, 16]. The relatively favourable prognosis for endometriosis-asso- ciated malignancies in comparison to primary ovarian cancer is explained, on the one hand, as a result of genetic mutations of en- dometriosis cells the former is pathogenetically a unique entity or, on the other hand, clinically that many carcinomas are diag- nosed and treated in the early stages. In the cases reported here, attack on the locoregional lymph nodes had already taken place; however, all 3 cases are still free of recurrences, in cases 2 and 3 even after almost 5 years which is rather in accord with a more favourable prognosis. Practical Conclusions ! In spite of its rare occurrence, the responsible physician should, in cases of peri- or postmenopausal patients with a history of en- dometriosis, when there is a clinical suspicion of an intestinal tu- mour in the lesser pelvis always take the possibility for a malig- nant transformation into consideration. Preoperative imaging procedures and biochemical tests are helpful for the interdiscipli- nary surgical planning but ultimately the suspected clinical and also intraoperative diagnosis can only be confirmed and man- aged on the basis of histological examinations. The objective is to encourage the pathologist to actively search for an atypical en- dometriosis and its malignant transformation and, in the case of women with a history of endometriosis and suspected intestinal adenocarcinoma, to employ the appropriate immunohistochem- ical examinations in the differential diagnosis. Exact knowledge of the origin is extremely important since a malignantly degener- ated intestinal endometriosis requires different adjuvant therapy procedures than a primary intestinal carcinoma and apparently has a better stage-dependent prognosis. Conflict of Interest ! None.

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