Exosomal miR-22-3p derived from peritoneal macrophages enhances proliferation, migration, and invasion of ectopic endometrial stromal cells through regulation of the SIRT1/NF-κB signaling pathway

L Zhang, H-H Li, M Yuan, D Li, G-Y Wang
European review for medical and pharmacological sciences · 2020 · doi:10.26355/eurrev_202001_20033 · PMID:32016958
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Exosomal miR-22-3p from peritoneal macrophages enhances ectopic endometrial stromal cell proliferation, migration, and invasion by targeting SIRT1 and activating the NF-κB pathway.

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⚙ AI-generated deep summary by claude@2026-06, 2026-06-09 · read from full text ⓘ

This paper investigated whether exosomes released by peritoneal macrophages (pMφ) from endometriosis patients deliver microRNAs to human ectopic endometrial stromal cells (eESCs), and which miRNAs mediate functional effects. Using differential centrifugation to isolate pMφ exosomes, confocal imaging to confirm delivery to eESCs, miRNA profiling (microarray and qRT-PCR), and functional assays (CCK-8, wound-healing, and transwell), the authors found that EMS pMφ-derived exosomes promoted eESC proliferation, migration, and invasion, with miR-22-3p being elevated and transferred. Mechanistically, bioinformatics and luciferase reporter assays indicated miR-22-3p targets SIRT1, and Western blot showed activation of the SIRT1/NF-κB pathway. This paper is centrally about endometriosis—specifically, exosomal miR-22-3p from peritoneal macrophages modulating eESC behavior via the SIRT1/NF-κB signaling pathway.

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Abstract

OBJECTIVE: Exosomes play crucial roles in cell-cell communication, but few studies exist on the role of exosomal miRNA in the interaction between peritoneal macrophages (pMφ) and human ectopic endometrial stromal cells (eESCs) in endometriosis (EMS). This study aimed to identify which exosomal miRNAs are significantly differently produced from EMS pMφ and to investigate the functional role of exosomal miRNAs in eESCs. PATIENTS AND METHODS: Exosomes were collected from the culture media of pMφ by differential centrifugation. Confocal microscopy was used to identify whether the exosomes secreted by pMφ can be delivered into eESCs. miRNA microarray and quantitative Reverse Transcription-Polymerase Chain Reaction (qRT-PCR) were used to identify which exosomal miRNAs were specifically elevated in pMφ-derived exosomes from EMS and delivered into eESCs via exosomes. The effect of pMφ-derived miR-22-3p on the biological function of eESCs was assessed by Cell Counting Kit-8 (CCK-8), wound-healing, and transwell chamber assays. Bioinformatics analysis and Luciferase reporter assay were used to detect the binding of exosomal miR-22-3p to the 3'untranslated region of SIRT1. Western blot was utilized to detect the activity of SIRT1/NF-κB pathway. RESULTS: Exosomes secreted by pMφ can successfully be transported to eESCs. pMφ-derived exosomes from EMS promoted the proliferation, migration, and invasion of eESCs. MiR-22-3p was significantly increased in pMφ-derived exosomes from EMS and delivered from pMφ to eESCs via exosomes. Mechanistic analyses revealed that exosomal miR-22-3p from pMφ promoted the proliferation, migration, and invasion of eESCs by targeting SIRT1 and activating NF-κB pathway. CONCLUSIONS: Exosomal miR-22-3p promotes the proliferation, migration, and invasion of eESCs by regulating SIRT1/NF-κB pathway and may serve as a novel target for the inhibition of EMS progression.
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Objective

Exosomes play crucial roles in cell-cell communication, but few studies exist on the role of exosomal miRNA in the interaction between peritoneal macrophages (pMφ) and human ectopic endometrial stromal cells (eESCs) in endometriosis (EMS). This study aimed to identify which exosomal miRNAs are significantly differently produced from EMS pMφ and to investigate the functional role of exosomal miRNAs in eESCs. PATIENTS AND METHODS: Exosomes were collected from the culture media of pMφ by differential centrifugation. Confocal microscopy was used to identify whether the exosomes secreted by pMφ can be delivered into eESCs. miRNA microarray and quantitative Reverse Transcription-Polymerase Chain Reaction (qRT-PCR) were used to identify which exosomal miRNAs were specifically elevated in pMφ-derived exosomes from EMS and delivered into eESCs via exosomes. The effect of pMφ-derived miR-22-3p on the biological function of eESCs was assessed by Cell Counting Kit-8 (CCK-8), wound-healing, and transwell chamber assays. Bioinformatics analysis and Luciferase reporter assay were used to detect the binding of exosomal miR-22-3p to the 3′untranslated region of SIRT1. Western blot was utilized to detect the activity of SIRT1/NF-κB pathway.

Results

Exosomes secreted by pMφ can successfully be transported to eESCs. pMφ-derived exosomes from EMS promoted the proliferation, migration, and invasion of eESCs. MiR-22-3p was significantly increased in pMφ-derived exosomes from EMS and delivered from pMφ to eESCs via exosomes. Mechanistic analyses revealed that exosomal miR-22-3p from pMφ promoted the proliferation, migration, and invasion of eESCs by targeting SIRT1 and activating NF-κB pathway.

Conclusions

Exosomal miR-22-3p promotes the proliferation, migration, and invasion of eESCs by regulating SIRT1/NF-κB pathway and may serve as a novel target for the inhibition of EMS progression. Free PDF DownloadThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License To cite this article L. Zhang, H.-H. Li, M. Yuan, D. Li, G.-Y. Wang Exosomal miR-22-3p derived from peritoneal macrophages enhances proliferation, migration, and invasion of ectopic endometrial stromal cells through regulation of the SIRT1/NF-κB signaling pathway Eur Rev Med Pharmacol Sci Year: 2020 Vol. 24 - N. 2 Pages: 571-580 DOI: 10.26355/eurrev_202001_20033 Publication History Published online: 30 Jan 2020

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Condition tags

endometriosis

MeSH descriptors

Endometriosis Exosomes Macrophages, Peritoneal MicroRNAs NF-kappa B Sirtuin 1 Cell Movement Cell Movement Cell Proliferation Cell Proliferation Cells, Cultured Endometriosis Endometriosis Exosomes Female Humans Macrophages, Peritoneal Macrophages, Peritoneal MicroRNAs MicroRNAs

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