Solid-phase microextraction of sweat components of patients positive for Sars-Cov-2 for identification of possible biomarkers

Solid-phase microextraction of sweat components of patients positive for Sars-Cov-2 for identification of possible biomarkers | Authorea try { document.documentElement.classList.add('js'); } catch (e) { } var _gaq = _gaq || []; _gaq.push(['_setAccount', 'G-8VDV14Y67G']); _gaq.push(['_trackPageview']); (function() { var ga = document.createElement('script'); ga.type = 'text/javascript'; ga.async = true; ga.src = ('https:' == document.location.protocol ? 'https://ssl' : 'http://www') + '.google-analytics.com/ga.js'; var s = document.getElementsByTagName('script')[0]; s.parentNode.insertBefore(ga, s); })(); Skip to main content Preprints Collections Wiley Open Research IET Open Research Ecological Society of Japan All Collections About About Authorea FAQs Contact Us Quick Search anywhere Search for preprint articles, keywords, etc. Search Search ADVANCED SEARCH SCROLL This is a preprint and has not been peer reviewed. Data may be preliminary. 9 May 2025 V1 Latest version Share on Solid-phase microextraction of sweat components of patients positive for Sars-Cov-2 for identification of possible biomarkers Authors : Lilia C. Soler-Jiménez 0000-0002-6784-7623 , Héctor A. Peniche-Pavía , Juan Vázquez Martínez , Emanuel Hernández-Nuñez , David Hernández-Mena , Juan Manuel Mancilla Tapia , Granja-Pérez P , and Víctor M. Vidal-Martínez [email protected] Authors Info & Affiliations https://doi.org/10.22541/au.174679398.85626259/v1 Published Scientific Reports Version of record Peer review timeline 169 views 89 downloads Contents Abstract Supplementary Material Information & Authors Metrics & Citations View Options References Figures Tables Media Share Abstract Metabolome is gaining consideration as a viable approach to disease detection and even shows promising results in COVID-19 diagnosis. This work extends the study of the relationship between solid-phase microextraction (SPME) extractable sweat compounds (SPME-ECs) and COVID-19 positive patients. Sweat samples were collected from 426 patients (126 positives and 300 negatives) recruited at Merida and Progreso (Yucatán, México) health centers. The composition of sweat was analyzed with a solid phase microextraction gas chromatography-mass spectrometry (SPME-GC-MS) method. The statistical analysis revealed significant differences between infected and non-infected patients’ SPME-EC profiles. Then, we evaluated different classification models to discriminate between positive and negative patients. The best model was the PLS-DA model, with percentages of specificity and sensitivity above 80%. Six relevant chromatographic peaks for the classification model were annotated (hexadecanoic acid, tetracosanoic acid, 6-hexadecenoic acid, oleic acid, squalene, and undecanal) based on the mass spectrum recognition using the NIST library (> 90% identity match). Palmitic and oleic acid signal was elevated in COVID-positive patients. While sapienic acid, lignoceric acid, squalene, and undecanal were detected in lower amount in COVID-positive patients. The present list of chemical opens the possibility to develop pseudo-smells for training dogs without exposing them to real life samples, which increases the risk of infection to the canids. Supplementary Material File (06052025 manuscript covid19_sweat_spme_gcms.docx) Download 191.96 KB Information & Authors Information Version history V1 Version 1 09 May 2025 Peer review timeline Published Scientific Reports Version of Record 13 Oct 2025 Published Copyright This work is licensed under a Non Exclusive No Reuse License. Keywords chromatographic techniques metabolic analysis research and analysis methods sars coronavirus virus classification Authors Affiliations Lilia C. Soler-Jiménez 0000-0002-6784-7623 Cinvestav Unidad Merida View all articles by this author Héctor A. Peniche-Pavía Cinvestav Unidad Merida View all articles by this author Juan Vázquez Martínez Instituto Tecnologico Superior de Irapuato View all articles by this author Emanuel Hernández-Nuñez Instituto Tecnologico Superior de Calkini View all articles by this author David Hernández-Mena Universidad Nacional Autonoma de Mexico View all articles by this author Juan Manuel Mancilla Tapia Centro de Biodeteccion OBI K9 View all articles by this author Granja-Pérez P Laboratorio Estatal de Salud Publica (LESP View all articles by this author Víctor M. Vidal-Martínez [email protected] Cinvestav Unidad Merida View all articles by this author Metrics & Citations Metrics Article Usage 169 views 89 downloads .FvxKWukQNSOunydq8rnd { width: 100px; } Citations Download citation Lilia C. Soler-Jiménez, Héctor A. Peniche-Pavía, Juan Vázquez Martínez, et al. Solid-phase microextraction of sweat components of patients positive for Sars-Cov-2 for identification of possible biomarkers. Authorea . 09 May 2025. DOI: https://doi.org/10.22541/au.174679398.85626259/v1 If you have the appropriate software installed, you can download article citation data to the citation manager of your choice. Simply select your manager software from the list below and click Download. For more information or tips please see 'Downloading to a citation manager' in the Help menu . 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