The Saliva Cortisol and Amylase Levels Related With Stress Response Compared by Different Analytical Methods

Abstract Sampling of salivary cortisol and salivary amylase is non-invasive and important for the evaluation of the hypothalamic-pituitar-adrenal axis funciton and stress levels. This study aims to compare the values of the cortisol and salivary amylase measured by using three diffeent analytical methods and discuss the comparison of stress levels of samples.Young adults (n =23) saliva samples were collected between 08.00 and 09.00 am, noon at 12.00 (before exam) and 14.00-15.00 (after unaware exam) during the first 48 hours, no freezing / thawing was done. Salivary cortisol and salivary amylase levels in subjects were measured by three different analytical methods (ELISA, Chemiluminescence and Biosensor).Comparison of method between ELISA and biosensor in determining salivary kortizol levels showed a good correlation y= 2.971+0.748x (R2=0.839). Salivary amylase concentrations were found only by ELISA method.The detection/information effectiveness as compared with conventional determination method ELISA, considering the low cost, fast results and specificity characteristics biosensor can be offered as an alternative method.