Study on the Differentiated Responses of Rhizosphere Soil and Microbial Communities of Eight Tree Species in Northern China to Ozone Stress

Study on the Differentiated Responses of Rhizosphere Soil and Microbial Communities of Eight Tree Species in Northern China to Ozone Stress | Authorea try { document.documentElement.classList.add('js'); } catch (e) { } var _gaq = _gaq || []; _gaq.push(['_setAccount', 'G-8VDV14Y67G']); _gaq.push(['_trackPageview']); (function() { var ga = document.createElement('script'); ga.type = 'text/javascript'; ga.async = true; ga.src = ('https:' == document.location.protocol ? 'https://ssl' : 'http://www') + '.google-analytics.com/ga.js'; var s = document.getElementsByTagName('script')[0]; s.parentNode.insertBefore(ga, s); })(); Skip to main content Preprints Collections Wiley Open Research IET Open Research Ecological Society of Japan All Collections About About Authorea FAQs Contact Us Quick Search anywhere Search for preprint articles, keywords, etc. Search Search ADVANCED SEARCH SCROLL This is a preprint and has not been peer reviewed. Data may be preliminary. 6 November 2025 V1 Latest version Share on Study on the Differentiated Responses of Rhizosphere Soil and Microbial Communities of Eight Tree Species in Northern China to Ozone Stress Authors : Qin Wang 0000-0001-6070-8148 [email protected] , Miao Li , Yiran Li 0009-0005-5513-7842 , Taotao Yu , Xiaoyi Zhou , Jianwei Ma , and Laiye Qu Authors Info & Affiliations https://doi.org/10.22541/au.176242755.56608422/v1 163 views 139 downloads Contents Abstract Supplementary Material Information & Authors Metrics & Citations View Options References Figures Tables Media Share Abstract Surface ozone (O3) is recognized as one of the most phytotoxic air pollutants. Using Open Top Chambers (OTCs) to simulate a high-ozone environment (60 ppb) relative to ambient air, this study investigated the effects of short-term O3 exposure on the composition and function of rhizosphere soil microbial communities of eight common tree species, as well as their relationships with nutrient factors. The results revealed that the structural and functional responses of rhizosphere microbial communities to O3 stress exhibited marked tree interspecific differences. Robinia pseudoacacia and Quercus mongolica exhibited the greatest changes in microbial community diversity and function, with O3 exposure markedly inhibiting the intracellular enzymatic activities of rhizosphere bacteria. Elevated O3 exerted stronger effects on the structure of the rhizosphere fungal community than on the bacterial community. Fungal community interactions were more sensitive to O3 stress. O3 fumigation increased the interspecific correlations in the fungal network by approximately 15.9%. O3 stress substantially increased the proportion of negative correlations among fungal species and reshaped fungal network connectivity. However, the bacterial network remained relatively stable. Therefore, the core microbial functional groups remained relatively stable coexistence under short-term O3 exposure. The disruptive effects of O3 were mainly concentrated on microbial functions related to nutrient cycling. The fungal community composition was significantly correlated with soil nitrogen availability. Overall, Robinia pseudoacacia and Quercus mongolica were identified as the most sensitive tree species to O3 stress. This study provides theoretical support for species adaptability management and microbial functional regulation in forest ecosystems under O3 pollution. Supplementary Material File (oik-12188-file001.doc) Download 14.18 MB File (oik-12188-file003.docx) Download 1.96 MB File (oik-12188-file004.docx) Download 1.69 MB File (oik-12188-file005.docx) Download 2.39 MB File (oik-12188-file006.docx) Download 6.23 MB File (oik-12188-file007.docx) Download 255.89 KB File (oik-12188-file008.docx) Download 1.07 MB Information & Authors Information Version history V1 Version 1 06 November 2025 Copyright This work is licensed under a Non Exclusive No Reuse License. Keywords microbial network connectivity nutrient cycling o₃ stress rhizosphere microbial community tree species sensitivity Authors Affiliations Qin Wang 0000-0001-6070-8148 [email protected] Hebei University View all articles by this author Miao Li Hebei University View all articles by this author Yiran Li 0009-0005-5513-7842 Hebei University View all articles by this author Taotao Yu Hebei University View all articles by this author Xiaoyi Zhou Hebei University View all articles by this author Jianwei Ma Hebei University View all articles by this author Laiye Qu State Key Laboratory of Urban and Regional Ecology View all articles by this author Metrics & Citations Metrics Article Usage 163 views 139 downloads .FvxKWukQNSOunydq8rnd { width: 100px; } Citations Download citation Qin Wang, Miao Li, Yiran Li, et al. Study on the Differentiated Responses of Rhizosphere Soil and Microbial Communities of Eight Tree Species in Northern China to Ozone Stress. Authorea . 06 November 2025. DOI: https://doi.org/10.22541/au.176242755.56608422/v1 If you have the appropriate software installed, you can download article citation data to the citation manager of your choice. Simply select your manager software from the list below and click Download. For more information or tips please see 'Downloading to a citation manager' in the Help menu . Format Please select one from the list RIS (ProCite, Reference Manager) EndNote BibTex Medlars RefWorks Direct import Tips for downloading citations document.getElementById('citMgrHelpLink').addEventListener('click', function() { popupHelp(this.href); return false; }); $(".js__slcInclude").on("change", function(e){ if ($(this).val() == 'refworks') $('#direct').prop("checked", false); $('#direct').prop("disabled", ($(this).val() == 'refworks')); }); View Options View options PDF View PDF Figures Tables Media Share Share Share article link Copy Link Copied! Copying failed. 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