Correlation and Biological Significance of miR-362 and LncRNA SNHG12 Expression Levels, IL-17 Methylation-Positive, and hr- HPV Infection in Patients with Cervical Cancer

Correlation and Biological Significance of miR-362 and LncRNA SNHG12 Expression Levels, IL-17 Methylation-Positive, and hr- HPV Infection in Patients with Cervical Cancer | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Article Correlation and Biological Significance of miR-362 and LncRNA SNHG12 Expression Levels, IL-17 Methylation-Positive, and hr- HPV Infection in Patients with Cervical Cancer Lijie He, Zongnan Chen, Heping Zhang This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-3990015/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract Objective To explore the correlation and biological significance between the expression levels of microribonucleic acid-362 (miR-362) and long-chain non coding ribonucleic acid (RNA) small nucleolar RNA host gene 12 (LncRNA SNHG12), the positive rate of interleukin-17 (IL-17) methylation, and high-risk human papillomavirus (hr-HPV) infection in patients with cervical cancer. Methods 168 patients with cervical cancer admitted to Tianjin Fifth Central Hospital from January 2019 to August 2023 were selected as the group A, 170 patients with cervical intraepithelial neoplasia admitted during the same period were selected as the group B, and 180 female volunteers who underwent health examinations during the same period were selected as the group C. The clinical data, rate of hr-HPV infection, expression levels of cervical tissue miR-362 and LncRNA SNHG12, positivity rate of IL-17 methylation of three groups, hr-HPV infection positive and expression levels of negative cervical tissue miR-362 and LncRNA SNHG12, positivity rate of IL-17 methylation were compared, and the correlation between expression levels of cervical tissue miR-362 and LncRNA SNHG12, positivity rate of IL-17 methylation and hr-HPV infection positivity in cervical cancer patients was analyzed by Spearman method, The expression levels of miR-362 and LncRNA SNHG12 in cervical tissues with different clinical and pathological characteristics, as well as the clinical and pathological characteristics of cervical cancer with different IL-17 methylation states were compared. Results The infection rates of hr-HPV in the group A, the group B, and the group C were 84.52%, 62.94%, and 7.78%, respectively, with statistically significant differences ( P < 0.05). The expression level of miR-362 in cervical tissue of the group A was lower than that of the group B and the group C, and the group B was lower than the group C; The expression level of LncRNA SNHG12 in cervical tissue of the group A was higher than that of the group B and the group C, and the group B was higher than the group C; The positive rates of IL-17 methylation in the three groups were 68.45%, 52.35%, and 37.78%, respectively, with statistically significant differences ( P < 0.05). The expression level of miR-362 in cervical tissue of patients with hr-HPV infection positive cervical cancer was lower than that of patients with hr-HPV infection negative cervical cancer; The expression level of LncRNA SNHG12 in cervical tissue was higher than that in patients with hr HPV infection negative cervical cancer; The positive rate of IL-17 methylation was 72.54%, which was higher than the 46.15% of patients with hr-HPV infection negative cervical cancer ( P < 0.05). The expression level of miR-362 in cervical tissue of cervical cancer patients was negatively correlated with positive hr HPV infection ( r =-0.565, P < 0.05); The expression level of LncRNA SNHG12 and the positivity rate of IL-17 methylation in cervical tissue were positively correlated with hr-HPV infection positivity ( r = 0.498, 0.512, P < 0.05). The expression levels of miR-362 and LncRNA SNHG12 in cervical tissue of the group A patients showed statistical differences ( P < 0.05) at different ages, levels of tumor differentiation, International Federation of Obstetrics and Gynecology (FIGO) staging, and lymph node metastasis. The proportion of highly differentiated tumors in IL-17 methylation positive patients was 60.87%, higher than the 39.62% in IL-17 methylation negative patients ( P < 0.05). Conclusion The expression levels of miR-362 and LncRNA SNHG12 in cervical tissue, as well as the positive rate of IL-17 methylation were closely related to the occurrence of cervical cancer and hr-HPV, and there was a certain correlation with the clinical and pathological characteristics of patients. Cervical cancer Microribonucleic acid-362 Long chain non-coding ribonucleic acid small nucleolar ribonucleic acid host gene 12 Interleukin-17 Methylation Relativity Full Text Additional Declarations No competing interests reported. Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-3990015","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Article","associatedPublications":[],"authors":[{"id":281246375,"identity":"b45fb6f0-dd44-4a4e-841c-cf159f067b95","order_by":0,"name":"Lijie He","email":"","orcid":"","institution":"Tianjin Fifth Central Hospital","correspondingAuthor":false,"prefix":"","firstName":"Lijie","middleName":"","lastName":"He","suffix":""},{"id":281246379,"identity":"4b2c5d24-5cf7-439d-8bb7-944973382964","order_by":1,"name":"Zongnan Chen","email":"","orcid":"","institution":"Tianjin Fifth Central Hospital","correspondingAuthor":false,"prefix":"","firstName":"Zongnan","middleName":"","lastName":"Chen","suffix":""},{"id":281246383,"identity":"ae1dc2ad-6313-42cd-b9f6-f7c5b804e7c2","order_by":2,"name":"Heping Zhang","email":"data:image/png;base64,iVBORw0KGgoAAAANSUhEUgAAAZAAAAAyAQMAAABI0h/eAAAABlBMVEX///8AAABVwtN+AAAACXBIWXMAAA7EAAAOxAGVKw4bAAAAzElEQVRIiWNgGAWjYBAC+/b2g49//vlX39jeQKQWA54zycaMDQcYm3sOEKtFIsFMGqSlfUYCkVrMGRISpAt33GHmnfl44w2GGptoglosGw4eMJ555hmb5Oy0YguGY2m5DQT1HGxISOBhY+YxnJ1jJsHYcJgILYcZDA4AtUjY3zxDpBaDYwyGzbxthw0YZ/AQqUWyhyeZccaZtATGHqBfEojxC7/88+M/PlTYJDC2H95440ONDRF+QXakRAIpyiFaSNUxCkbBKBgFIwMAADVURScC6YbPAAAAAElFTkSuQmCC","orcid":"","institution":"Tianjin Fifth Central Hospital","correspondingAuthor":true,"prefix":"","firstName":"Heping","middleName":"","lastName":"Zhang","suffix":""}],"badges":[],"createdAt":"2024-02-26 05:30:12","currentVersionCode":1,"declarations":"","doi":"10.21203/rs.3.rs-3990015/v1","doiUrl":"https://doi.org/10.21203/rs.3.rs-3990015/v1","draftVersion":[],"editorialEvents":[],"editorialNote":"","failedWorkflow":false,"files":[{"id":59823619,"identity":"65c577e6-093f-45df-a768-15811e500129","added_by":"auto","created_at":"2024-07-08 04:59:40","extension":"pdf","order_by":1,"title":"","display":"","copyAsset":false,"role":"manuscript-pdf","size":358952,"visible":true,"origin":"","legend":"","description":"","filename":"miR362LncRNASNHG12andIL17forhrHPV.pdf","url":"https://assets-eu.researchsquare.com/files/rs-3990015/v1_covered_8e58b359-0e97-4146-9be7-674befe48040.pdf"}],"financialInterests":"No competing interests reported.","formattedTitle":"Correlation and Biological Significance of miR-362 and LncRNA SNHG12 Expression Levels, IL-17 Methylation-Positive, and hr- HPV Infection in Patients with Cervical Cancer","fulltext":[],"fulltextSource":"","fullText":"","funders":[],"hasAdminPriorityOnWorkflow":false,"hasManuscriptDocX":false,"hasOptedInToPreprint":true,"hasPassedJournalQc":"","hasAnyPriority":false,"hideJournal":true,"highlight":"","institution":"","isAcceptedByJournal":false,"isAuthorSuppliedPdf":true,"isDeskRejected":"","isHiddenFromSearch":false,"isInQc":false,"isInWorkflow":false,"isPdf":true,"isPdfUpToDate":true,"isWithdrawnOrRetracted":false,"journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true},"keywords":"Cervical cancer, Microribonucleic acid-362, Long chain non-coding ribonucleic acid small nucleolar ribonucleic acid host gene 12, Interleukin-17, Methylation, Relativity","lastPublishedDoi":"10.21203/rs.3.rs-3990015/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-3990015/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"\u003ch2\u003eObjective\u003c/h2\u003e \u003cp\u003eTo explore the correlation and biological significance between the expression levels of microribonucleic acid-362 (miR-362) and long-chain non coding ribonucleic acid (RNA) small nucleolar RNA host gene 12 (LncRNA SNHG12), the positive rate of interleukin-17 (IL-17) methylation, and high-risk human papillomavirus (hr-HPV) infection in patients with cervical cancer.\u003c/p\u003e\u003ch2\u003eMethods\u003c/h2\u003e \u003cp\u003e168 patients with cervical cancer admitted to Tianjin Fifth Central Hospital from January 2019 to August 2023 were selected as the group A, 170 patients with cervical intraepithelial neoplasia admitted during the same period were selected as the group B, and 180 female volunteers who underwent health examinations during the same period were selected as the group C. The clinical data, rate of hr-HPV infection, expression levels of cervical tissue miR-362 and LncRNA SNHG12, positivity rate of IL-17 methylation of three groups, hr-HPV infection positive and expression levels of negative cervical tissue miR-362 and LncRNA SNHG12, positivity rate of IL-17 methylation were compared, and the correlation between expression levels of cervical tissue miR-362 and LncRNA SNHG12, positivity rate of IL-17 methylation and hr-HPV infection positivity in cervical cancer patients was analyzed by \u003cem\u003eSpearman\u003c/em\u003e method, The expression levels of miR-362 and LncRNA SNHG12 in cervical tissues with different clinical and pathological characteristics, as well as the clinical and pathological characteristics of cervical cancer with different IL-17 methylation states were compared.\u003c/p\u003e\u003ch2\u003eResults\u003c/h2\u003e \u003cp\u003eThe infection rates of hr-HPV in the group A, the group B, and the group C were 84.52%, 62.94%, and 7.78%, respectively, with statistically significant differences (\u003cem\u003eP\u003c/em\u003e\u0026thinsp;\u0026lt;\u0026thinsp;0.05). The expression level of miR-362 in cervical tissue of the group A was lower than that of the group B and the group C, and the group B was lower than the group C; The expression level of LncRNA SNHG12 in cervical tissue of the group A was higher than that of the group B and the group C, and the group B was higher than the group C; The positive rates of IL-17 methylation in the three groups were 68.45%, 52.35%, and 37.78%, respectively, with statistically significant differences (\u003cem\u003eP\u003c/em\u003e\u0026thinsp;\u0026lt;\u0026thinsp;0.05). The expression level of miR-362 in cervical tissue of patients with hr-HPV infection positive cervical cancer was lower than that of patients with hr-HPV infection negative cervical cancer; The expression level of LncRNA SNHG12 in cervical tissue was higher than that in patients with hr HPV infection negative cervical cancer; The positive rate of IL-17 methylation was 72.54%, which was higher than the 46.15% of patients with hr-HPV infection negative cervical cancer (\u003cem\u003eP\u003c/em\u003e\u0026thinsp;\u0026lt;\u0026thinsp;0.05). The expression level of miR-362 in cervical tissue of cervical cancer patients was negatively correlated with positive hr HPV infection (\u003cem\u003er\u003c/em\u003e=-0.565, \u003cem\u003eP\u003c/em\u003e\u0026thinsp;\u0026lt;\u0026thinsp;0.05); The expression level of LncRNA SNHG12 and the positivity rate of IL-17 methylation in cervical tissue were positively correlated with hr-HPV infection positivity (\u003cem\u003er\u003c/em\u003e\u0026thinsp;=\u0026thinsp;0.498, 0.512, \u003cem\u003eP\u003c/em\u003e\u0026thinsp;\u0026lt;\u0026thinsp;0.05). The expression levels of miR-362 and LncRNA SNHG12 in cervical tissue of the group A patients showed statistical differences (\u003cem\u003eP\u003c/em\u003e\u0026thinsp;\u0026lt;\u0026thinsp;0.05) at different ages, levels of tumor differentiation, International Federation of Obstetrics and Gynecology (FIGO) staging, and lymph node metastasis. The proportion of highly differentiated tumors in IL-17 methylation positive patients was 60.87%, higher than the 39.62% in IL-17 methylation negative patients (\u003cem\u003eP\u003c/em\u003e\u0026thinsp;\u0026lt;\u0026thinsp;0.05).\u003c/p\u003e\u003ch2\u003eConclusion\u003c/h2\u003e \u003cp\u003eThe expression levels of miR-362 and LncRNA SNHG12 in cervical tissue, as well as the positive rate of IL-17 methylation were closely related to the occurrence of cervical cancer and hr-HPV, and there was a certain correlation with the clinical and pathological characteristics of patients.\u003c/p\u003e","manuscriptTitle":"Correlation and Biological Significance of miR-362 and LncRNA SNHG12 Expression Levels, IL-17 Methylation-Positive, and hr- HPV Infection in Patients with Cervical Cancer","msid":"","msnumber":"","nonDraftVersions":[{"code":1,"date":"2024-03-20 18:36:58","doi":"10.21203/rs.3.rs-3990015/v1","editorialEvents":[{"type":"communityComments","content":0}],"status":"published","journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true}}],"origin":"","ownerIdentity":"c0f2bb78-cdde-480a-bb13-7769cebe090d","owner":[],"postedDate":"March 20th, 2024","published":true,"recentEditorialEvents":[],"rejectedJournal":[],"revision":"","amendment":"","status":"posted","subjectAreas":[],"tags":[],"updatedAt":"2024-07-08T04:51:33+00:00","versionOfRecord":[],"versionCreatedAt":"2024-03-20 18:36:58","video":"","vorDoi":"","vorDoiUrl":"","workflowStages":[]},"version":"v1","identity":"rs-3990015","journalConfig":"researchsquare"},"__N_SSP":true},"page":"/article/[identity]/[[...version]]","query":{"redirect":"/article/rs-3990015","identity":"rs-3990015","version":["v1"]},"buildId":"qtupq5eGEP_6zYnWcrvyt","isFallback":false,"isExperimentalCompile":false,"dynamicIds":[84888],"gssp":true,"scriptLoader":[]}