The Association between Metformin treatment in type 2 DM and vitamin B12 Deficiency

The Association between Metformin treatment in type 2 DM and vitamin B12 Deficiency | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article The Association between Metformin treatment in type 2 DM and vitamin B12 Deficiency Sahar H. Mahmoud, Fatma Elfargani, Nagwa Mohamed, Fiaza A. Alhamdi This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-5271037/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract Background: Background: Diabetes mellitus (DM) is a serious health condition affecting people worldwide with a high prevalence rate. DM is classified according to its etiology into T1DM and T2DM; several risk factors contribute to the development of T2DM such as age, genetic predisposition, and obesity. Metformin is a proven drug of choice, and it is used as a first-line treatment for T2DM patients to improve their glycemic levels. Vitamin B12 is an important water-soluble vitamin, and its deficiency can lead to megaloblastic anemia. Aims of the study: To determine the prevalence of diabetic patients and patients under metformin treatment, the level of vitamin B12 for patients who have T2DM and the correlation between vitamin B12 deficiency, metformin dose, and the duration of T2DM are measured. Methodology: A retrospective study was conducted on 366 patients with T2DM in a Sedi Hussein diabetic polyclinic in Benghazi- Libya. Patients were divided into two groups: the control group (not on metformin) consisted of 71 patients, and the group that received metformin (1st group) consisted of 295 patients. Results: The total sample was 381 patients; T1DM 15 patients (3.94%), and T2DM 366 (96.06%) patients. In T2DM The first group had 295 patients (117 male/178 female), and the control group had 71 (28 male / 43 female). The 1st group significantly decreased vitamin B12 serum levels by 443.56 mg/ml versus the control group of 541.33 pg/ml (p=0.003). There was no significant difference in correlation between serum vitamin B12 levels and both doses of metformin and duration of diabetes mellitus. Conclusion: vitamin B12 was significantly decreased in T2DM patients receiving metformin. T2DM Metformin vitamin B12 levels Full Text Additional Declarations No competing interests reported. Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-5271037","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":377363628,"identity":"5b83990f-5edb-4d2b-9c69-48db7841989f","order_by":0,"name":"Sahar H. 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DM is classified according to its etiology into T1DM and T2DM; several risk factors contribute to the development of T2DM such as age, genetic predisposition, and obesity. Metformin is a proven drug of choice, and it is used as a first-line treatment for T2DM patients to improve their glycemic levels. Vitamin B12 is an important water-soluble vitamin, and its deficiency can lead to megaloblastic anemia.\u0026nbsp;\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eAims of the study:\u003c/strong\u003e To determine the prevalence of diabetic patients and patients under metformin treatment, the level of vitamin B12 for patients who have T2DM and the correlation between vitamin B12 deficiency, metformin dose, and the duration of T2DM are measured.\u0026nbsp;\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eMethodology:\u003c/strong\u003e A retrospective study was conducted on 366 patients with T2DM in a Sedi Hussein diabetic polyclinic in Benghazi- Libya. Patients were divided into two groups: the control group (not on metformin) consisted of 71 patients, and the group that received metformin (1st group) consisted of 295 patients.\u0026nbsp;\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eResults:\u003c/strong\u003e The total sample was 381 patients; T1DM 15 patients (3.94%), and T2DM 366 (96.06%) patients. In T2DM The first group had 295 patients (117 male/178 female), and the control group had 71 (28 male / 43 female). The 1st group significantly decreased vitamin B12 serum levels by 443.56 mg/ml versus the control group of 541.33 pg/ml (p=0.003). There was no significant difference in correlation between serum vitamin B12 levels and both doses of metformin and duration of diabetes mellitus.\u0026nbsp;\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eConclusion:\u003c/strong\u003e vitamin B12 was significantly decreased in T2DM patients receiving metformin.\u003c/p\u003e","manuscriptTitle":"The Association between Metformin treatment in type 2 DM and vitamin B12 Deficiency","msid":"","msnumber":"","nonDraftVersions":[{"code":1,"date":"2024-11-13 05:27:42","doi":"10.21203/rs.3.rs-5271037/v1","editorialEvents":[{"type":"communityComments","content":0}],"status":"published","journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true}}],"origin":"","ownerIdentity":"bc9b8d67-185e-4ec5-86f0-2a62a1c02a29","owner":[],"postedDate":"November 13th, 2024","published":true,"recentEditorialEvents":[],"rejectedJournal":[],"revision":"","amendment":"","status":"posted","subjectAreas":[],"tags":[],"updatedAt":"2024-11-26T07:08:24+00:00","versionOfRecord":[],"versionCreatedAt":"2024-11-13 05:27:42","video":"","vorDoi":"","vorDoiUrl":"","workflowStages":[]},"version":"v1","identity":"rs-5271037","journalConfig":"researchsquare"},"__N_SSP":true},"page":"/article/[identity]/[[...version]]","query":{"redirect":"/article/rs-5271037","identity":"rs-5271037","version":["v1"]},"buildId":"qtupq5eGEP_6zYnWcrvyt","isFallback":false,"isExperimentalCompile":false,"dynamicIds":[84888],"gssp":true,"scriptLoader":[]}