Field Dissipation, Dietary Risk Assessment, and Soil Fate of Chlorantraniliprole and Abamectin (Dual-Active Formulation) in Watermelon under Field Conditions

Field Dissipation, Dietary Risk Assessment, and Soil Fate of Chlorantraniliprole and Abamectin (Dual-Active Formulation) in Watermelon under Field Conditions | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Article Field Dissipation, Dietary Risk Assessment, and Soil Fate of Chlorantraniliprole and Abamectin (Dual-Active Formulation) in Watermelon under Field Conditions Bhaidas Deore, Yogesh Saindane, Durgesh Narwade, Sachin Aher, and 5 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-8500769/v1 This work is licensed under a CC BY 4.0 License Status: Published Journal Publication published 11 Apr, 2026 Read the published version in Scientific Reports → Version 1 posted 19 You are reading this latest preprint version Abstract Chlorantraniliprole and abamectin are widely applied together for pest management in cucurbit crops; however, comprehensive field residue data for their combined use on watermelon remain absent. This study characterizes the dissipation dynamics, dietary safety profile, and environmental fate of chlorantraniliprole and abamectin following application of a dual-active formulation (Voliam Targo; 4.3% + 1.7% SC) to watermelon under supervised field conditions. Fruit samples were collected at 0, 1, 3, 5, 7, 10, and 15 days after final spray; soil samples were collected at harvest. Residues were analyzed by LC-MS/MS following a modified QuEChERS extraction with matrix-matched calibration. The method demonstrated excellent analytical performance: linearity R² > 0.995, recovery 94.8-107.8%, and precision (RSD) < 8%. At the recommended dose, initial residues were 0.081 mg/kg (chlorantraniliprole) and 0.125 mg/kg (avermectin B1a); both declined below their respective limits of quantification (0.03 and 0.05 mg/kg) within 7–10 days following first-order exponential decay kinetics (half-lives: 3.79–4.1 days for chlorantraniliprole; 6.7–7.5 days for abamectin). Soil analysis at harvest revealed no detectable residues, indicating minimal environmental transfer. Dietary risk assessment showed chlorantraniliprole posed a negligible risk (hazard quotient = 0.002), while abamectin residues were safe when realistic pre-harvest intervals were applied (HQ declined from 2.08 at day 0 to 0.52 at day 7). These integrated findings, combining rapid dissipation, soil safety, and dietary risk characterization, establish a scientific basis for a 7-10day pre-harvest interval and support in establishing crop-specific maximum residue limits and pre-harvest intervals for watermelon. Earth and environmental sciences/Environmental sciences Biological sciences/Plant sciences chlorantraniliprole abamectin watermelon dissipation kinetics dietary risk assessment LC-MS/MS Full Text Additional Declarations No competing interests reported. Supplementary Files Supplemetrymaterial.docx Cite Share Download PDF Status: Published Journal Publication published 11 Apr, 2026 Read the published version in Scientific Reports → Version 1 posted Editorial decision: Revision requested 13 Feb, 2026 Reviews received at journal 12 Feb, 2026 Reviews received at journal 12 Feb, 2026 Reviewers agreed at journal 12 Feb, 2026 Reviews received at journal 11 Feb, 2026 Reviewers agreed at journal 11 Feb, 2026 Reviewers agreed at journal 11 Feb, 2026 Reviewers agreed at journal 11 Feb, 2026 Reviewers agreed at journal 11 Feb, 2026 Reviewers agreed at journal 10 Feb, 2026 Reviewers agreed at journal 09 Feb, 2026 Reviewers agreed at journal 09 Feb, 2026 Reviewers agreed at journal 09 Feb, 2026 Reviewers agreed at journal 09 Feb, 2026 Reviewers invited by journal 09 Feb, 2026 Editor invited by journal 07 Jan, 2026 Editor assigned by journal 03 Jan, 2026 Submission checks completed at journal 03 Jan, 2026 First submitted to journal 02 Jan, 2026 You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-8500769","acceptedTermsAndConditions":true,"allowDirectSubmit":false,"archivedVersions":[],"articleType":"Article","associatedPublications":[],"authors":[{"id":589097330,"identity":"208b7576-bc46-4208-a3c2-529c2b62ffb0","order_by":0,"name":"Bhaidas Deore","email":"","orcid":"","institution":"MPKV","correspondingAuthor":false,"prefix":"","firstName":"Bhaidas","middleName":"","lastName":"Deore","suffix":""},{"id":589097331,"identity":"488824bf-2910-4162-83f1-57023c5bac53","order_by":1,"name":"Yogesh Saindane","email":"","orcid":"","institution":"MPKV","correspondingAuthor":false,"prefix":"","firstName":"Yogesh","middleName":"","lastName":"Saindane","suffix":""},{"id":589097332,"identity":"ab42f8ae-1047-47c0-9afd-436d8fa132a4","order_by":2,"name":"Durgesh Narwade","email":"data:image/png;base64,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","orcid":"","institution":"MPKV","correspondingAuthor":true,"prefix":"","firstName":"Durgesh","middleName":"","lastName":"Narwade","suffix":""},{"id":589097333,"identity":"bfa9ec21-700a-45fe-97bf-7d2ae4f7af62","order_by":3,"name":"Sachin Aher","email":"","orcid":"","institution":"MPKV","correspondingAuthor":false,"prefix":"","firstName":"Sachin","middleName":"","lastName":"Aher","suffix":""},{"id":589097334,"identity":"65d55ea6-509f-4939-bf76-97ee3c397433","order_by":4,"name":"Kunal Magar","email":"","orcid":"","institution":"MPKV","correspondingAuthor":false,"prefix":"","firstName":"Kunal","middleName":"","lastName":"Magar","suffix":""},{"id":589097335,"identity":"df18f67d-d9cc-4775-af00-f97fe61f6853","order_by":5,"name":"Jayant Nawale","email":"","orcid":"","institution":"MPKV","correspondingAuthor":false,"prefix":"","firstName":"Jayant","middleName":"","lastName":"Nawale","suffix":""},{"id":589097336,"identity":"86d2ce6e-6bed-4e08-bf00-fed1f4f282a7","order_by":6,"name":"Suvarna Deore","email":"","orcid":"","institution":"MPKV","correspondingAuthor":false,"prefix":"","firstName":"Suvarna","middleName":"","lastName":"Deore","suffix":""},{"id":589097340,"identity":"58c4eb6b-0b27-44f3-a937-15f6bad7b507","order_by":7,"name":"Uttam Kadam","email":"","orcid":"","institution":"MPKV","correspondingAuthor":false,"prefix":"","firstName":"Uttam","middleName":"","lastName":"Kadam","suffix":""},{"id":589097342,"identity":"007dd81d-40a4-45db-8353-5fe32d06b348","order_by":8,"name":"Satappa Kharbade","email":"","orcid":"","institution":"MPKV","correspondingAuthor":false,"prefix":"","firstName":"Satappa","middleName":"","lastName":"Kharbade","suffix":""}],"badges":[],"createdAt":"2026-01-02 12:09:16","currentVersionCode":1,"declarations":"","doi":"10.21203/rs.3.rs-8500769/v1","doiUrl":"https://doi.org/10.21203/rs.3.rs-8500769/v1","draftVersion":[],"editorialEvents":[{"content":"https://doi.org/10.1038/s41598-026-46390-4","type":"published","date":"2026-04-11T15:59:31+00:00"}],"editorialNote":"","failedWorkflow":false,"files":[{"id":106810154,"identity":"6fcf53b9-4d48-42fb-bdd1-82c9b83c08ef","added_by":"auto","created_at":"2026-04-13 16:14:32","extension":"pdf","order_by":1,"title":"","display":"","copyAsset":false,"role":"manuscript-pdf","size":890072,"visible":true,"origin":"","legend":"","description":"","filename":"Manuscript.pdf","url":"https://assets-eu.researchsquare.com/files/rs-8500769/v1_covered_03af1c95-a054-46a6-8d85-b389dfc16cff.pdf"},{"id":102421250,"identity":"c72e16a9-2962-40ec-8cb5-0d72582eb7c3","added_by":"auto","created_at":"2026-02-11 13:44:58","extension":"docx","order_by":1,"title":"","display":"","copyAsset":false,"role":"supplement","size":22154,"visible":true,"origin":"","legend":"","description":"","filename":"Supplemetrymaterial.docx","url":"https://assets-eu.researchsquare.com/files/rs-8500769/v1/e536f47d4b3f64091d4ba108.docx"}],"financialInterests":"No competing interests reported.","formattedTitle":"Field Dissipation, Dietary Risk Assessment, and Soil Fate of Chlorantraniliprole and Abamectin (Dual-Active Formulation) in Watermelon under Field Conditions","fulltext":[],"fulltextSource":"","fullText":"","funders":[],"hasAdminPriorityOnWorkflow":false,"hasManuscriptDocX":false,"hasOptedInToPreprint":true,"hasPassedJournalQc":"","hasAnyPriority":false,"hideJournal":false,"highlight":"","institution":"","isAcceptedByJournal":true,"isAuthorSuppliedPdf":true,"isDeskRejected":"","isHiddenFromSearch":false,"isInQc":false,"isInWorkflow":false,"isPdf":true,"isPdfUpToDate":true,"isWithdrawnOrRetracted":false,"journal":{"display":true,"email":"[email protected]","identity":"scientific-reports","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":false,"externalIdentity":"scirep","sideBox":"Learn more about [Scientific Reports](http://www.nature.com/srep/)","snPcode":"","submissionUrl":"","title":"Scientific Reports","twitterHandle":"","acdcEnabled":true,"dfaEnabled":true,"editorialSystem":"stoa","reportingPortfolio":"Scientific Reports","inReviewEnabled":true,"inReviewRevisionsEnabled":true},"keywords":"chlorantraniliprole, abamectin, watermelon, dissipation kinetics, dietary risk assessment, LC-MS/MS","lastPublishedDoi":"10.21203/rs.3.rs-8500769/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-8500769/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"\u003cp\u003eChlorantraniliprole and abamectin are widely applied together for pest management in cucurbit crops; however, comprehensive field residue data for their combined use on watermelon remain absent. This study characterizes the dissipation dynamics, dietary safety profile, and environmental fate of chlorantraniliprole and abamectin following application of a dual-active formulation (Voliam Targo; 4.3% + 1.7% SC) to watermelon under supervised field conditions. Fruit samples were collected at 0, 1, 3, 5, 7, 10, and 15 days after final spray; soil samples were collected at harvest. Residues were analyzed by LC-MS/MS following a modified QuEChERS extraction with matrix-matched calibration. The method demonstrated excellent analytical performance: linearity R\u0026sup2; \u0026gt; 0.995, recovery 94.8-107.8%, and precision (RSD)\u0026thinsp;\u0026lt;\u0026thinsp;8%. At the recommended dose, initial residues were 0.081 mg/kg (chlorantraniliprole) and 0.125 mg/kg (avermectin B1a); both declined below their respective limits of quantification (0.03 and 0.05 mg/kg) within 7\u0026ndash;10 days following first-order exponential decay kinetics (half-lives: 3.79\u0026ndash;4.1 days for chlorantraniliprole; 6.7\u0026ndash;7.5 days for abamectin). Soil analysis at harvest revealed no detectable residues, indicating minimal environmental transfer. Dietary risk assessment showed chlorantraniliprole posed a negligible risk (hazard quotient\u0026thinsp;=\u0026thinsp;0.002), while abamectin residues were safe when realistic pre-harvest intervals were applied (HQ declined from 2.08 at day 0 to 0.52 at day 7). These integrated findings, combining rapid dissipation, soil safety, and dietary risk characterization, establish a scientific basis for a 7-10day pre-harvest interval and support in establishing crop-specific maximum residue limits and pre-harvest intervals for watermelon.\u003c/p\u003e","manuscriptTitle":"Field Dissipation, Dietary Risk Assessment, and Soil Fate of Chlorantraniliprole and Abamectin (Dual-Active Formulation) in Watermelon under Field Conditions","msid":"","msnumber":"","nonDraftVersions":[{"code":1,"date":"2026-02-11 13:44:53","doi":"10.21203/rs.3.rs-8500769/v1","editorialEvents":[{"type":"communityComments","content":0},{"type":"decision","content":"Revision requested","date":"2026-02-13T09:04:12+00:00","index":"","fulltext":""},{"type":"editorInvitedReview","content":"","date":"2026-02-12T21:58:07+00:00","index":"hide","fulltext":""},{"type":"editorInvitedReview","content":"","date":"2026-02-12T09:28:32+00:00","index":"hide","fulltext":""},{"type":"reviewerAgreed","content":"235404501792730270026051976190988694156","date":"2026-02-12T09:07:38+00:00","index":"hide","fulltext":""},{"type":"editorInvitedReview","content":"","date":"2026-02-12T04:31:08+00:00","index":"hide","fulltext":""},{"type":"reviewerAgreed","content":"294665043606284788177391027349082417264","date":"2026-02-12T03:27:45+00:00","index":"hide","fulltext":""},{"type":"reviewerAgreed","content":"168033731427404414220343051926185547978","date":"2026-02-11T16:10:58+00:00","index":"hide","fulltext":""},{"type":"reviewerAgreed","content":"109527637872287537198458617199581353302","date":"2026-02-11T15:33:03+00:00","index":"hide","fulltext":""},{"type":"reviewerAgreed","content":"21447227258712238507920235064365662191","date":"2026-02-11T07:42:12+00:00","index":"hide","fulltext":""},{"type":"reviewerAgreed","content":"107668767975132725128380384469354836613","date":"2026-02-10T08:36:06+00:00","index":"hide","fulltext":""},{"type":"reviewerAgreed","content":"9215080985904744826904920005341902653","date":"2026-02-10T00:58:02+00:00","index":"hide","fulltext":""},{"type":"reviewerAgreed","content":"68191158707462154295179563951298372779","date":"2026-02-09T18:02:03+00:00","index":"hide","fulltext":""},{"type":"reviewerAgreed","content":"245965924936038065042187828423492852517","date":"2026-02-09T16:09:58+00:00","index":"hide","fulltext":""},{"type":"reviewerAgreed","content":"139646673967178784371208256577510236121","date":"2026-02-09T15:29:05+00:00","index":"hide","fulltext":""},{"type":"reviewersInvited","content":"","date":"2026-02-09T15:10:37+00:00","index":"","fulltext":""},{"type":"editorInvited","content":"","date":"2026-01-07T07:53:33+00:00","index":"","fulltext":""},{"type":"editorAssigned","content":"","date":"2026-01-03T12:11:54+00:00","index":"","fulltext":""},{"type":"checksComplete","content":"","date":"2026-01-03T12:10:39+00:00","index":"","fulltext":""},{"type":"submitted","content":"Scientific Reports","date":"2026-01-02T11:54:50+00:00","index":"","fulltext":""}],"status":"published","journal":{"display":true,"email":"[email protected]","identity":"scientific-reports","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":false,"externalIdentity":"scirep","sideBox":"Learn more about [Scientific Reports](http://www.nature.com/srep/)","snPcode":"","submissionUrl":"","title":"Scientific Reports","twitterHandle":"","acdcEnabled":true,"dfaEnabled":true,"editorialSystem":"stoa","reportingPortfolio":"Scientific Reports","inReviewEnabled":true,"inReviewRevisionsEnabled":true}}],"origin":"","ownerIdentity":"de2cbfed-5528-4c6b-9ee7-730e5bacf6ce","owner":[],"postedDate":"February 11th, 2026","published":true,"recentEditorialEvents":[],"rejectedJournal":[],"revision":"","amendment":"","status":"published-in-journal","subjectAreas":[{"id":62680616,"name":"Earth and environmental sciences/Environmental sciences"},{"id":62680617,"name":"Biological sciences/Plant sciences"}],"tags":[],"updatedAt":"2026-04-13T16:10:41+00:00","versionOfRecord":{"articleIdentity":"rs-8500769","link":"https://doi.org/10.1038/s41598-026-46390-4","journal":{"identity":"scientific-reports","isVorOnly":false,"title":"Scientific Reports"},"publishedOn":"2026-04-11 15:59:31","publishedOnDateReadable":"April 11th, 2026"},"versionCreatedAt":"2026-02-11 13:44:53","video":"","vorDoi":"10.1038/s41598-026-46390-4","vorDoiUrl":"https://doi.org/10.1038/s41598-026-46390-4","workflowStages":[]},"version":"v1","identity":"rs-8500769","journalConfig":"researchsquare"},"__N_SSP":true},"page":"/article/[identity]/[[...version]]","query":{"redirect":"/article/rs-8500769","identity":"rs-8500769","version":["v1"]},"buildId":"XKTyCvWXoU3ODBz1xrDgd","isFallback":false,"isExperimentalCompile":false,"dynamicIds":[84888],"gssp":true,"scriptLoader":[]}