Analysis of the Digestion Dynamics and Dietary Risk Assessment of Fluridone in Cotton Fields via QuEChERS Coupled with HPLC

Fluridone is a pyrrolidone soil-sealing herbicide that has been widely used in cotton fields in Xinjiang in recent years. The purpose of this study was to establish a method for determining fluridone residues in cotton fields and to perform residue digestion tests, final residue analysis, and dietary risk assessment. Samples were extracted with acetonitrile, purified with PSA and MWCNTs, and analysed by HPLC. The results showed that in a certain concentration range, the concentration and peak area of fluridone showed a good linear relationship (R2>0.99), with LOD value and LOQ values of 0.0090-0.0108 mg·kg⁻¹ and 0.0030-0.0033 mg·kg⁻¹, respectively. The relative standard deviation (RSD) values of fluridone were 0.46% to 4.57% at the spiked level of 0.1, 0.5 and 1.0 mg·kg⁻¹, respectively. The average daily recovery rate of fluridone was 85.08% to 95.07%. The residual levels of fluridone in cottonseed oil were below the safety threshold, indicating no significant dietary risk to consumers.