DIFFERENTIATION OF DENDRITIC CELLS WITH RANTES AND IL-12 ENHANCES THE ACTION OF GAMMA DELTA T LYMPHOCYTES IN AN EXPERIMENTAL BREAST CANCER MODEL

Purpose: Breast cancer, a leading cause of death in female cancer types, often poses a high metastasis risk. The study focuses on enhancing antitumor immunotherapy using dendritic cells (DCs), vital for initiating and coordinating adaptive immune responses. Additionally, gamma delta (γδ) T lymphocytes, known for their cytokine secretion (Interleukin 17 [IL-17], Interferon gamma [IFN-γ]) and cytotoxicity against various cells, are examined. Methods: To evaluate the effectiveness of different DC maturation protocols, female BALB/c mice were divided into three groups: Tumor (n=12), Protocol 1 (n=12, maturation with TNF-α), and Protocol 2 (n=12, maturation with TNF-α, IL-12, and RANTES). All were induced to develop tumors using the 4T1 cell line, followed by treatment with either Protocol 1 or 2. The quantification of cytotoxic T lymphocytes (CD8+) producing IFN-γ and γδ T lymphocytes producing IL-17 and IFN-γ was conducted using flow cytometry. Results: Findings revealed a higher number of cytotoxic T lymphocytes producing IFN-γ and γδ T lymphocytes producing IL-17 and IFN-γ in mice treated with Protocol 2 compared to Protocol 1. This suggests Protocol 2’s superior efficacy in DC maturation for antitumor treatment. Conclusion: Protocol 2 demonstrates greater efficiency in DC maturation for antitumor purposes. However, being an experimental study, further research is necessary to fully understand the optimal dendritic cell maturation protocol and the role of IL-17 and IFN-γ cytokines in combating tumor cells. Supplementary Material File (manuscript.docx) - Download - 48.20 KB Information & Authors Information Version history Copyright This work is licensed under a Non Exclusive No Reuse License.

Authors Metrics & Citations Metrics Article Usage 175views 95downloads Citations Download citation Cláudio JOSÉ SILVA, Marcia Antoniazi Michelin. DIFFERENTIATION OF DENDRITIC CELLS WITH RANTES AND IL-12 ENHANCES THE ACTION OF GAMMA DELTA T LYMPHOCYTES IN AN EXPERIMENTAL BREAST CANCER MODEL. Authorea. 07 July 2025. DOI: https://doi.org/10.22541/au.175185915.50413660/v1 DOI: https://doi.org/10.22541/au.175185915.50413660/v1 If you have the appropriate software installed, you can download article citation data to the citation manager of your choice. Simply select your manager software from the list below and click Download. For more information or tips please see 'Downloading to a citation manager' in the Help menu.