Hyperosmotic induction enables rapid and synchronized larval release in the brooding coral Pocillopora damicornis

Hyperosmotic induction enables rapid and synchronized larval release in the brooding coral Pocillopora damicornis | Authorea try { document.documentElement.classList.add('js'); } catch (e) { } var _gaq = _gaq || []; _gaq.push(['_setAccount', 'G-8VDV14Y67G']); _gaq.push(['_trackPageview']); (function() { var ga = document.createElement('script'); ga.type = 'text/javascript'; ga.async = true; ga.src = ('https:' == document.location.protocol ? 'https://ssl' : 'http://www') + '.google-analytics.com/ga.js'; var s = document.getElementsByTagName('script')[0]; s.parentNode.insertBefore(ga, s); })(); Skip to main content Preprints Collections Wiley Open Research IET Open Research Ecological Society of Japan All Collections About About Authorea FAQs Contact Us Quick Search anywhere Search for preprint articles, keywords, etc. Search Search ADVANCED SEARCH SCROLL Ecology and Evolution This is a preprint and has not been peer reviewed. Data may be preliminary. 12 May 2026 V1 Latest version Share on Hyperosmotic induction enables rapid and synchronized larval release in the brooding coral Pocillopora damicornis Authors : Zhuqing Wang 0000-0001-6450-138X [email protected] , Danyang Wang [email protected] , Chengyue Liu [email protected] , Shumin Zhang [email protected] , Shaowen Ke [email protected] , Jingjie Hu [email protected] , and Zhenmin Bao [email protected] Authors Info & Affiliations https://doi.org/10.22541/authorea.15003170/v1 18 views 11 downloads Contents Abstract Supplementary Material Information & Authors Metrics & Citations View Options References Figures Tables Media Share Abstract Coral reefs, vital marine ecosystems, are undergoing rapid degradation, highlighting the urgent need for effective restoration strategies. The brooding coral Pocillopora damicornis, characterized by high reproductive frequency and environmental adaptability, is considered a potential pioneer species for reef recovery. However, the unpredictability of natural larval release and the lack of reliable induction methods limit the availability of larvae for both research and restoration applications. Here, we developed a hyperosmotic induction protocol that enables rapid and synchronized larval release through controlled salinity manipulation. Under this regime, colonies released larvae within 15-20 h post-induction with minimal observable stress, and induced larvae exhibited high settlement success (84.3% within 35-40 h). Leveraging these larvae, we conducted genome-wide SNP genotyping, which revealed the presence of two genetically distinguishable larval groups differing in relatedness patterns and genomic composition. Overall, our results demonstrate that hyperosmotic induction provides a scalable and minimally invasive approach for controlled larval release, enabling reliable access to early life stages. Coupled with genome-wide analyses, this framework offers new opportunities to investigate larval genetic diversity and its ecological implications, with potential applications in coral research, aquaculture, and reef restoration. Supplementary Material File (supplementary tables.xlsx) supplementary tables Download 144.77 KB Information & Authors Information Version history V1 Version 1 12 May 2026 Collection Ecology and Evolution Authors Affiliations Zhuqing Wang 0000-0001-6450-138X [email protected] View all articles by this author Danyang Wang [email protected] View all articles by this author Chengyue Liu [email protected] Southern Marine Science and Engineering Guangdong Laboratory - Guangzhou, Guangzhou, China, 511458 View all articles by this author Shumin Zhang [email protected] Southern Marine Science and Engineering Guangdong Laboratory - Guangzhou, Guangzhou, China, 511458 View all articles by this author Shaowen Ke [email protected] Southern Marine Science and Engineering Guangdong Laboratory - Guangzhou, Guangzhou, China, 511458 View all articles by this author Jingjie Hu [email protected] View all articles by this author Zhenmin Bao [email protected] View all articles by this author Metrics & Citations Metrics Article Usage 18 views 11 downloads .FvxKWukQNSOunydq8rnd { width: 100px; } Citations Download citation Zhuqing Wang, Danyang Wang, Chengyue Liu, et al. Hyperosmotic induction enables rapid and synchronized larval release in the brooding coral Pocillopora damicornis. Authorea . 12 May 2026. DOI: https://doi.org/10.22541/authorea.15003170/v1 If you have the appropriate software installed, you can download article citation data to the citation manager of your choice. Simply select your manager software from the list below and click Download. For more information or tips please see 'Downloading to a citation manager' in the Help menu . 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