Revealing More with Less: Dose Efficiency in Electron Imaging

Revealing More with Less: Dose Efficiency in Electron Imaging | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Article Revealing More with Less: Dose Efficiency in Electron Imaging Anastasios Pateras, Luc J. Bourhis, Roger Durst, Niels de Jonge, and 1 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-6649593/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract Single-particle cryogenic electron microscopy (cryo-EM) has experienced remarkable advancements in the past twenty years, emerging as a powerful technique for solving molecular structures at sub-4 Å resolutions critical for atomic model building. However, challenges persist due to low scattering contrast in biomolecules and their radiation sensitivity, necessitating low-dose, low-signal imaging and the averaging of thousands of noisy, randomly oriented particle images. Here, we compare the dose efficiency of plane-wave electron coherent diffractive imaging (CDI), ptychography, and high-resolution transmission electron microscopy (HRTEM) on weak-phase biological molecules by simulating two-dimensional images and estimating their resolution with the use of the Fourier ring correlation (FRC) as a function of electron dose. Our results illustrate the potential for adopting ptychography in single-particle cryo-EM for the next generation of electron microscopes for molecular imaging. Biological sciences/Biotechnology Physical sciences/Materials science Physical sciences/Physics electron dose coherent electron diffractive imaging electron ptychography phase-retrieval high-resolution transmission electron microscopy Full Text Additional Declarations No competing interests reported. Supplementary Files Paterasetal.npjComputationalMaterials030725SI.docx Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-6649593","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Article","associatedPublications":[],"authors":[{"id":463382274,"identity":"bb9aebfc-fe5f-419c-bc13-8182a000559e","order_by":0,"name":"Anastasios Pateras","email":"data:image/png;base64,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","orcid":"","institution":"Deutsches Elektronen-Synchrotron DESY","correspondingAuthor":true,"prefix":"","firstName":"Anastasios","middleName":"","lastName":"Pateras","suffix":""},{"id":463382275,"identity":"4e81368e-ffbe-4e67-b80d-a4341bf7bedd","order_by":1,"name":"Luc J. 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