CENPF Upregulation is Associated with Immunosuppressive Status and Predicts Poor Clinical Outcomes in Lung Adenocarcinoma Validated by Qrt- PCR

Background: CENPF was differentially expressed in various cancers. However, the relationship between CENPF and immune infiltrates in lung adenocarcinoma was previously unknown. Methods: : We implemented a comprehensive analysis of expression of CENPF in the GEO and TCGA databases. CENPF was evaluated for its prognostic value combining clinical samples from the GEPIA2 and TCGA databases. The Metascape together with WebGestalt databases were used for enrichment analysis of genesets that were most postively associated with CENPF. We retrieved the score for immune cell infiltration in TCGA data and examined the correlation between CENPF expression and the infiltration of immune cell by R software. Results: : The outcomes exhibited that up-regulated CENPF mRNA expression was evidently related to poor PFS, DSS and OS in patients with lung adenocarcinoma. Moreover, high expression of CENPF was markedly related to genes associated with immune checkpoint. Further analysis showed that T cell CD4+ Th2 infiltration increased in lung adenocarcinoma samples with high CENPF expression. Conclusions: : Our study indicated that CENPF led to T cell CD4+ Th2 infiltration through oncogenic activity and may be employed as a biomarker for the prediction of prognosis in lung adenocarcinoma.