Proteomic Analysis of Stromal and Epithelial Cell Communications in Human Endometrial Cancer Using a Unique 3D Co‐Culture Model

Aminah Ali Abid Al-Juboori, Aminah Ali Abid Al‐Juboori, Arnab Ghosh, M. Fairuz B. Jamaluddin, Muhammad Fairuz Bin Jamaluddin, Kumar M, Manish Kumar, Subhransu Sekhar Sahoo, Subhransu S. Sahoo, Shafiq M. Syed, Shafiq Mukhtar Syed, Nahar P, Pravin Nahar, Tanwar PS, Pradeep Singh Tanwar, Pradeep S. Tanwar
Proteomics · 2019 · vol. 19(21-22) , pp. e1800448 · doi:10.1002/pmic.201800448 · PMID:30865368 · W2921981992
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A novel 3D co-culture model of human endometrial cancer spheroids was developed, revealing 591 common proteins with in vivo tissue and demonstrating drug screening feasibility.

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Abstract

Epithelial and stromal communications are essential for normal uterine functions and their dysregulation contributes to the pathogenesis of many diseases including infertility, endometriosis, and cancer. Although many studies have highlighted the advantages of culturing cells in 3D compared to the conventional 2D culture system, one of the major limitations of these systems is the lack of incorporation of cells from non-epithelial lineages. In an effort to develop a culture system incorporating both stromal and epithelial cells, 3D endometrial cancer spheroids are developed by co-culturing endometrial stromal cells with cancerous epithelial cells. The spheroids developed by this method are phenotypically comparable to in vivo endometrial cancer tissue. Proteomic analysis of the co-culture spheroids comparable to human endometrial tissue revealed 591 common proteins and canonical pathways that are closely related to endometrium biology. To determine the feasibility of using this model for drug screening, the efficacy of tamoxifen and everolimus is tested. In summary, a unique 3D model system of human endometrial cancer is developed that will serve as the foundation for the further development of 3D culture systems incorporating different cell types of the human uterus for deciphering the contributions of non-epithelial cells present in cancer microenvironment.

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Condition tags

endometriosisinfertility

MeSH descriptors

Cell Communication Cell Communication Coculture Techniques Endometrial Neoplasms Endometrial Neoplasms Epithelial Cells Epithelial Cells Proteomics Cell Line, Tumor Cell Proliferation Cell Proliferation Endometrial Neoplasms Endometrium Endometrium Endometrium Endometrium Epithelial Cells Epithelial Cells Everolimus Everolimus

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