Neuron stress-related genes serve as newbiomarkers in hypothalamic tissue following high fat diet

Abstract Objective Energy homeostasis is modulated by the hypothalamic is essential for obesity progression; however, the gene expression profiling remains to be fully understood. Methods GEO datasets were downloaded from the GEO website and analysed by the R packages to obtain the DEGs. And, the WGCNA analysis and PPI networks of co-expressed DEGs were designed using STRING to get key genes. In addition, the single-cell sequencing datasets and GTEx database were utilized to receive the neuron-stress genes from the key genes. Further, HFD-induced hypothalamic tissue of mice was used as an animal model to validate the mRNA up-regulation of neuron-stress genes. Finally, the Bmi1 gene was identified as a hub gene through the LASSO model and nomogram analysis. Results It found that six genes (Sacm1l, Junb, Bmi1, Erbb4, Dkc1, and Suv39h1) are neuron stress-related genes and increased in the HFD-induced mice obesity model. Finally, Bmi1gene was identified as hub genes that can reflect the pathophysiology of obesity. Conclusions Our research depicted a comprehensive activation map of cell abnormality in the obese hypothalamus and Bim1 may be a diagnostic marker in the clinic, which provides a new perspective and basis for investigating the pathogenesis of obesity.