Examining the Impact of Ventilation Systems Under Various Pressures on Lab Contamination During the Extraction of COVID-19 RNA

Objectives: The goal of this project was to evaluate the degree of contamination of the air and lab surfaces during the extraction of RNA from COVID-19 under laboratory ventilation systems, identify operational risk areas, and provide a reference for reducing medical contamination in COVID-19 detection.Methods: Our COVID-19 testing contains the real-world test and a splash test, detected the RNA residue of COVID-19 pseudovirus on the surface of air and object with PCR.Results: Our results show that RNA extraction contamination occurs more frequently under non-negative pressure than under negative pressure. The biological safety cabinet features a system to stop contamination that may successfully halt the transmission of aerosols as well as the contamination of the experimenter's glove, which is a major source of transmission. Furthermore, UV disinfection cannot completely sterilize an area and regular and effective cleaning is also necessary.Conclusions: In summary,our study lays the foundation for efficacy evaluation of laboratory construction of positive and negative pressure systems and also elucidates the focus of laboratory contamination prevention, providing a strong anti-contamination basis for the extraction of RNA testing for the prevention and control of COVID-19 and even other respiratory transmitted diseases.