Small interference RNA targeting NF-κB inhibits human endometriotic growth in nude mice

Objective To investigate the processes regulated by NF-κB in the initial development of endometriotic lesions in vivo by means of siRNA targeting NF-κB interfering with the growth of endometrium.Methods Menstrual endometrium of women with endometriosis was injected into the peritoneal cavities of 30 nude mice.NF-κB p65 siRNA was given intraperitoneally on days 3 and 6,and mice were killed.The number,diameter and morphometry of endometriotic lesions were quantified.NF-κB,vascular epithelial growth factor(VEGF) and intercellular adhesion molecule(ICAM)-1 expression were evaluated by immunohistochemical analyses.Results NF-κB p65 siRNA was significantly reduced in lesion development compared to control mice.Endometrial gland grew immaturely in treated mice.In addition,NF-κB activation,VEGF and ICAM-1 expression of endometriotic lesions were significantly reduced.Conclusion NF-κB pathway is involved in the initial development of endometriosis in vivo,and may serve as a target for endometriosis treatment.