Crebinostat Facilitates Memory Formation

Objectives Protein modifications importantly contribute to memory formation. Protein acetylation is a posttranslational modification of proteins that regulates memory formation. Acetylation level is determined by the relative activities of acetylases and deacetylases. Crebinostat is a histone deacetylase inhibitor. In this study, our aim was to examine whether Crebinostat affects memory formation in object recognition task. Further, we aimed to understand regulation of plasticity-related proteins by Crebinostat. Methods Object recognition task was used to examine the effect of Crebinostat on memory formation. The effect of Crebinostat on plasticity-related proteins was examined using Western blot experiments. Results We show that Crebinostat facilitates memory formation by a weak training in object recognition task. Further, this compound enhances acetylation of α-tubulin, and reduces the level of histone deacetylase 6, an α-tubulin deacetylase. Conclusions The results suggest that enhanced acetylation of α-tubulin by Crebinostat contributes to its facilitatory effect on memory formation. Protein modifications importantly contribute to memory formation. Protein acetylation is a posttranslational modification of proteins that regulates memory formation. Acetylation level is determined by the relative activities of acetylases and deacetylases. Crebinostat is a histone deacetylase inhibitor. Here we show that in object recognition task, Crebinostat facilitates memory formation by a weak training. Further, this compound enhances acetylation of α-tubulin, and reduces the level of histone deacetylase 6, an α-tubulin deacetylase. The results suggest that enhanced acetylation of α-tubulin by Crebinostat contributes to its facilitatory effect on memory formation.