Metabolomics Study of Patients with Chronic Heart Failure Based on Nuclear Magnetic Resonance

Objective: To determine the metabolic changes accompanying heart failure and identify novel diagnostic biomarkers. Methods: : Plasma samples were collected from 100 healthy controls and 200 heart failure patients who were admitted to the First Affiliated Hospital of Xinjiang Medical University from January 2021 to January 2022. The samples were analyzed by proton nuclear magnetic resonance. The differential metabolites among the three groups were identified by principal component analysis and orthogonal partial least squares discriminant analysis(OPLS-DA), and the correlation between metabolite concentration and heart failure was evaluated. Results: : Compared to the healthy control group, the serum levels of 3-hydroxybutyrate, alanine, citrulline and acetone were significantly higher in the HFmrEF and HFrEF groups ( P <0.05). Furthermore, these metabolites were present in significantly higher concentrations in the HFrEF compared to the HFmrEF group ( P <0.05). Conclusion: The heart failure patients have a distinct metabolome, and 3-hydroxybutyric acid, alanine, citrulline and acetone are potential biomarkers of cardiac dysfunction. Metabolomics can provide a reliable diagnostic method for heart failure.