Application of Microdissection in Molecular Biology Study of Endometriosis

Objective: Our aim was to evaluate the microdissection in molecular biology study of endometriosis. Methods: Forty-six cases of endometriosis were selected with hysterectomy and focus-resection during operation. Tissues with ectopic focus were embedded with paraffin blocks, sliced as usual (7 μm ) , and stained with HE. Then ectopic endo-metrium were found and labeled. The paraffin blocks were serially sliced again ( 15 μm ). The eetopic endometrium on corresponding sites of every section were scrapped off with knife and collected in the same tube for further use. The fresh ectopic endometrium were as control. DNA of each group were extracted by using phenol-chlorin extraction method and amplified by using PCR. The PCR products were electrophorcd on 2% agarose gel. Results: Among the total 46 cases, 22 ca-ses(47.83%) were with ectopic endometrium of which 63.64% were in the 1 to II stage, 42.86% in III to IV stage. The electrophoresis bands of the test group were as clear as those of the control group. Conclusion: The ectopic of endometrium could be got accurately and the results of studying were scientific and credible.