DNA Chisel, a versatile sequence optimizer

Motivation Accounting for biological and practical requirements in DNA sequence design often results in challenging optimization problems. Current software solutions are problem-specific and hard to combine. Results DNA Chisel is an easy-to-use, easy-to-extend sequence optimization framework allowing to freely define and combine optimization specifications via Python scripts or Genbank annotations. Availability as a web application ( https://cuba.genomefoundry.org/sculpt_a_sequence ) or open-source Python library (code and documentation at https://github.com/Edinburgh-Genome-Foundry/DNAChisel ). Contact [email protected] Supplementary information attached.