Preparation, Physicochemical Properties, Invitro And In Vivo Release Evaluation of Chitosan Decorated Curcumin Loaded Niosome

Abstract Purpose: Based on several pharmacological activities of curcumin (Cn), it has been introduced as an ideal candidate for different neurological disorders. But poor solubility, rapid clearance and low stability have limited its clinical application. Development of curcumin loaded smart niosome for crossing blood brain barrier could be and interesting option to overcome these limitations.Object: The aim of this study is preparation and characterization of chitosan decorated curcumin loaded niosome(CH-CLN) and evaluation of invitro release and in vivo bioavailability and bio stability of curcumin in the brain tissue.Methods: Niosomal formulations were prepared by modified heating method by using 3-factor and 3 level mixture design. The formulations were characterized using DLS, zeta potential, Fourier transform infrared (FTIR) spectroscopy, high performance liquid chromatography (HPLC) and transmission electron microscopy(TEM). The entrapment efficiency and invitro release were also evaluated. Wistar rats were subjected to intraperitoneal injection (i. p.) of formulations, and curcumin 15 minutes before perfusion. Cn concentration in different parts of the central nervous system, liver, and plasma was analyzed for in vivo analysis.Results: Encapsulation efficiency was obtained 75% for optimized formulation and presented sustain release, followed by the Hixon-Crowell model. The particle size was about 100nm with polydispersity index (PDI) of 0.2. Chitosan decorated niosomal formulation increased Cn concentration in central nervous system.Conclusion: We can conclude the chitosan decorated curcumin loaded niosome improved bioavailability of curcumin in brain tissue and cold be a promising tool for crossing blood-brain barrier