Higher copy number of an Arachis hypogaea glutamate receptor-like candidate gene is associated with increased resistance to Tomato spotted wilt virus

Higher copy number of an Arachis hypogaea glutamate receptor-like candidate gene is associated with increased resistance to Tomato spotted wilt virus | Authorea try { document.documentElement.classList.add('js'); } catch (e) { } var _gaq = _gaq || []; _gaq.push(['_setAccount', 'G-8VDV14Y67G']); _gaq.push(['_trackPageview']); (function() { var ga = document.createElement('script'); ga.type = 'text/javascript'; ga.async = true; ga.src = ('https:' == document.location.protocol ? 'https://ssl' : 'http://www') + '.google-analytics.com/ga.js'; var s = document.getElementsByTagName('script')[0]; s.parentNode.insertBefore(ga, s); })(); Skip to main content Preprints Collections Wiley Open Research IET Open Research Ecological Society of Japan All Collections About About Authorea FAQs Contact Us Quick Search anywhere Search for preprint articles, keywords, etc. Search Search ADVANCED SEARCH SCROLL This is a preprint and has not been peer reviewed. Data may be preliminary. 2 April 2026 V1 Latest version Share on Higher copy number of an Arachis hypogaea glutamate receptor-like candidate gene is associated with increased resistance to Tomato spotted wilt virus Authors : Sydney Webb , Ye Chu 0000-0002-8977-7945 , Akshaya Biswal 0000-0002-5652-6007 , Walid Korani , Stephanie Botton , Sameer Pokhrel , Ethan Thompson , Baozhu Guo 0000-0002-9079-7126 , Albert Culbreath , Josh Clevenger , Corley Holbrook 0000-0002-7668-0378 , and Peggy Ozias-Akins 0000-0002-8864-2766 [email protected] Authors Info & Affiliations https://doi.org/10.22541/au.177516495.50413723/v1 82 views 39 downloads Contents Abstract Supplementary Material Information & Authors Metrics & Citations View Options References Figures Tables Media Share Abstract Orthotospovirus tomatomaculae (Tomato spotted wilt virus, TSWV) is one of the most destructive viruses of the peanut crop, with significant economic consequences. Genetic resistance is desired to prevent major yield losses for growers. SSD6 and its progeny NC94022 have been introduced as resistance sources for breeding new varieties. Genetic evaluation of NC94022-derived populations previously reported multiple quantitative trait loci (QTLs) for resistance on chromosome Arahy.01. Subsequently, an insertion region from NC94022 and its parent SSD6, was found from whole genome sequencing. A unique quadrupled glutamate receptor like ( GLR ) gene was identified within the insertion region as a potential candidate gene. Eight populations were derived from crosses made between a single recombinant inbred line (RIL) containing the insertion region and four copies of the GLR gene, and eight unique parents without those. Field evaluation over three generations revealed a significant increase in TSWV resistance when the insertion region was present with high GLR copy number. All populations showed improved resistance compared with susceptible varieties and their unique parents, while only a few were as resistant as NC94022 or the RIL parent, perhaps due to GLR gene copy number variation due to early generation heterozygosity. These results suggest that improved TSWV resistance from SSD6 or NC94022 may be most efficiently selected by screening for higher GLR gene copy number. Supplementary Material File (tswvresistanceqtlvalidation_submitted.docx) Download 592.53 KB Information & Authors Information Version history V1 Version 1 02 April 2026 Copyright This work is licensed under a Non Exclusive No Reuse License. Keywords disease resistance groundnut peanut tswv Authors Affiliations Sydney Webb University of Georgia Tifton Campus View all articles by this author Ye Chu 0000-0002-8977-7945 University of Georgia Tifton Campus View all articles by this author Akshaya Biswal 0000-0002-5652-6007 University of Georgia Tifton Campus View all articles by this author Walid Korani HudsonAlpha Institute for Biotechnology View all articles by this author Stephanie Botton University of Georgia Tifton Campus View all articles by this author Sameer Pokhrel University of Georgia Tifton Campus View all articles by this author Ethan Thompson University of Georgia View all articles by this author Baozhu Guo 0000-0002-9079-7126 USDA-ARS View all articles by this author Albert Culbreath University of Georgia View all articles by this author Josh Clevenger HudsonAlpha Institute for Biotechnology View all articles by this author Corley Holbrook 0000-0002-7668-0378 USDA-ARS View all articles by this author Peggy Ozias-Akins 0000-0002-8864-2766 [email protected] University of Georgia Tifton Campus View all articles by this author Funding Information National Institute of Food and Agriculture 2019-67013-30365 Peggy Ozias-Akins Metrics & Citations Metrics Article Usage 82 views 39 downloads .FvxKWukQNSOunydq8rnd { width: 100px; } Citations Download citation Sydney Webb, Ye Chu, Akshaya Biswal, et al. Higher copy number of an Arachis hypogaea glutamate receptor-like candidate gene is associated with increased resistance to Tomato spotted wilt virus. Authorea . 02 April 2026. DOI: https://doi.org/10.22541/au.177516495.50413723/v1 If you have the appropriate software installed, you can download article citation data to the citation manager of your choice. Simply select your manager software from the list below and click Download. For more information or tips please see 'Downloading to a citation manager' in the Help menu . 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