Activity of Natural Substances and Alkyl Glycosides Against Formation of Pathogenic Biofilms by Pseudomonas aeruginosa

Biofilms of uropathogenic bacteria, especially biofilms of Pseudomonas aeruginosa due to the appliance of medical devices such as urinary catheters, cause complications in the treatment of urinary tract infections (UTI). Therefore, the search for efficient control options for biofilms is of great medical interest. This work aims to estimate the use of natural substances as an alternative to antibiotics to mitigate the formation of biofilms also regarding the spreading resistance of uropathogenic bacteria to antibiotics. In this work, due to the complexity of its biofilms, P. aeruginosa ATCC 27853 and P. aeruginosa PA01 were chosen as test organisms. Various natural substances and alkyl glycosides were investigated for their influence on biofilm formation by P. aeruginosa. Biofilm growth on catheter surfaces was determined by the number of colony-forming units per mL(CFU/mL). N-undecyl-α/β-l-fucopyranoside inhibited biofilm formation in P. aeruginosa ATCC 27853, whereas a slight increase in biofilm formation was observed in P. aeruginosa PA01. Significant inhibition of P. aeruginosa biofilm formation was observed with the detergents Soluplus®, Prontosan®, and the fungal metabolite terrein.