mAFiA: Detecting m6A at single-molecular resolution via direct-RNA sequencing

Direct-RNA sequencing offers the possibility to simultaneously identify canonical bases and epi-transcriptomic modifications in each single RNA molecule. Thus far, the development of computational methods has been hampered by the lack of biologically realistic training data that carries modification labels at molecular resolution. Here, we report on the synthesis of such samples and the development of a bespoke algorithm that accurately detects single m 6 A nucleotides on single molecules in both synthetic RNAs and natural mRNA.