Development of the “EASY-HSV (Efficient And Simple high-Yield Herpes Simplex virus Vector) system” based on HSV-1 genome-maintaining HEK293 cells

Herpes simplex virus (HSV) amplicon vector is a promising molecular tool with a large transgene capacity of 150 kb. However, the conventional vector production method is labor-intensive and expensive, limiting its application. Here, we generated HEK293 cells harboring the HSV genome (293/HSV) and developed an EASY-HSV (Efficient And Simple high-Yield Herpes Simplex virus Vector) system in which 293/HSV cells are transfected with only two plasmids.