Determination of 650nm wavelength for rapid detection of sickle cell anemia

Abstract Background Sickle cell anemia (SCA), is a type of inherited hemoglobin diseases, It is resulting from point mutation in the β-hemoglobin gene, that resulted from replacement of adenine with thymine, producing a phenotype of sickle hemoglobin (HbS). This paper represents a first try of hemo-photodiagnosis for such disorders using single wavelength, that absorbed by normal blood, so that any change in absorbance value interprets as pathological disorders of hemoglobin. Methods The proposed photobiosensor was designed according to Beer-Lamberts law. The system was involved diode laser of 650nm wavelength, with 100 mW output power, where the laser beam passed through a cuvette of blood sample, and then transmitted light emite to an optical lens to focus transmitted laser beam in to 2mm in diameter of laser spot size on optical sensor window of laser power meter detector that measure the amount of transmitted light in volts. Results The readings that displayed on LCD of laser power meter, had been analyzed using compare means values and ANOVA table of SPSS program. Conclusion The main conclusion that photobiosensor design was able to distinguish between experimental blood groups with respect to hemoglobin pathological conditions.