An open-sourced 3D printable microscope with a large CNC stage

3D printed laboratory tools are a fast-developing field, and microscopes in particular have seen several high-quality projects reported in recent years. However, currently available projects for 3D printed microscopes have not been optimised for molecular biology applications. We present an open-source, 3D-printable optical microscope that has been specifically designed for affordability, ease of use, and versatility in molecular biology applications, particularly for fluorescence detection, time lapse imaging, and higher throughput scanning of microtitre plates. The microscope integrates a large computer numerically controlled (CNC) stage, allowing precise scanning of wells in standard microtiter plates, and is equipped with a Raspberry Pi HQ camera module and customizable optical components, including standard microscope objectives. The modular design allows for a range of illumination setups, such as a 3 W blue LED for basic epifluorescence, and supports additional modifications, including dark field or RGB matrix lighting. The design opens new possibilities for cheap home-built microscopes for bioscience applications that are modular and easily adaptable for specific user needs.