Development of a fully automated workstation for conducting routine SABRE hyperpolarization

Development of a fully automated workstation for conducting routine SABRE hyperpolarization | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Article Development of a fully automated workstation for conducting routine SABRE hyperpolarization Jing Yang, Ruodong Xin, Sören Lehmkuhl, Jan G. Korvink, Jürgen J. Brandner This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-4570900/v1 This work is licensed under a CC BY 4.0 License Status: Published Journal Publication published 09 Sep, 2024 Read the published version in Scientific Reports → Version 1 posted 10 You are reading this latest preprint version Abstract SABRE is emerging as a fast, simple and low-cost hyperpolarization method because of its ability to regenerate enhanced NMR signals. Generally, SABRE hyperpolarization has been performed predominantly manually, leading to variations in reproducibility and efficiency. Recent advances in SABRE include the development of automated shuttling systems to address previous inconsistencies. However, the operational complexity of such systems and the challenges of integration with existing workflows hinder their widespread adoption. This work presents a fully automated lab workstation based on a benchtop NMR spectrometer, specifically designed to facilitate SABRE of different nuclei across different polarization fields. We demonstrated the capability of this system through a series of routine SABRE experimental protocols, including consecutive SABRE hyperpolarization with high reproducibility (average standard deviation of 1.03%), optimization polarization of 13C nuclei respect to the polarization transfer field, and measurement of polarization buildup rate or decay time across a wide range of magnetic fields. Furthermore, we have iteratively optimized the durations for pulsed SABRE-SHEATH 13C pyruvate.The constructed SABRE workstation offers full automation, high reproducibility, and functional diversification, making it a practical tool for conducting routine SABRE hyperpolarization experiments. It provides a robust platform for high-throughput and reliable SABRE and X-SABRE hyperpolarization studies. Physical sciences/Chemistry Physical sciences/Engineering Full Text Additional Declarations No competing interests reported. Cite Share Download PDF Status: Published Journal Publication published 09 Sep, 2024 Read the published version in Scientific Reports → Version 1 posted Editorial decision: Revision requested 21 Jul, 2024 Reviews received at journal 17 Jul, 2024 Reviewers agreed at journal 08 Jul, 2024 Reviews received at journal 08 Jul, 2024 Reviewers agreed at journal 29 Jun, 2024 Reviewers invited by journal 27 Jun, 2024 Editor assigned by journal 19 Jun, 2024 Editor invited by journal 18 Jun, 2024 Submission checks completed at journal 14 Jun, 2024 First submitted to journal 12 Jun, 2024 You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. 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Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-4570900","acceptedTermsAndConditions":true,"allowDirectSubmit":false,"archivedVersions":[],"articleType":"Article","associatedPublications":[],"authors":[{"id":320845344,"identity":"464719b2-e279-4da6-b8e3-2bf421a9feab","order_by":0,"name":"Jing Yang","email":"","orcid":"","institution":"Karlsruhe Institute of Technology (KIT), Institute of Microstructure Technology (IMT)","correspondingAuthor":false,"prefix":"","firstName":"Jing","middleName":"","lastName":"Yang","suffix":""},{"id":320845347,"identity":"a942668b-258b-4dfb-bafc-93e5588d5bd9","order_by":1,"name":"Ruodong Xin","email":"","orcid":"","institution":"Karlsruhe Institute of Technology (KIT), Institute of Microstructure Technology (IMT)","correspondingAuthor":false,"prefix":"","firstName":"Ruodong","middleName":"","lastName":"Xin","suffix":""},{"id":320845348,"identity":"d86799e0-ac61-43ee-8550-b0207af32f1f","order_by":2,"name":"Sören Lehmkuhl","email":"","orcid":"","institution":"Karlsruhe Institute of Technology (KIT), Institute of Microstructure Technology (IMT)","correspondingAuthor":false,"prefix":"","firstName":"Sören","middleName":"","lastName":"Lehmkuhl","suffix":""},{"id":320845349,"identity":"82a17cef-0f21-4460-b705-f1e9caa9938a","order_by":3,"name":"Jan G. 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