Interaction between TGF-β1 and long non-coding RNA 4.9 in a model of latent infection with human cytomegalovirus

To investigate the interaction between long non-coding RNA4.9 (lncRNA4.9) and transforming growth factor-β1 (TGF-β1) in the model of HCMV DNA. The experiment was divided into 5 groups: NC group, PCDNA-lncRNA4.9 group, SH-lncRNA4.9 group, LV-TGF-β1) Group, and SH-lncRNA4.9 group. Compared with the NC group, overexpression of lncRNA4.9 could significantly increase ( P <0.05) the expression of TGF-β1 and decrease with time. The relative expression of HCMV DNA was significantly decreased ( P <0.05) with the overexpression of lncRNA4.9. Compared with the NC group, overexpression of TGF-β1 significantly increased the expression of lncRNA4.9 and decreased with time. The relative expression of HCMV DNA was significantly decreased ( P <0.05) with the overexpression of TGF-β1. However, silencing TGF-β1 could not reduce the relative expression of lncRNA4.9, but increased the relative expression of lncRNA4.9 on day 5, and the difference was statistically significant ( P <0.05). Silencing lncRNA4.9 could not reduce expression of TGF-β1, but increased expression of lncRNA4.9 on day 5 ( P <0.05). Overexpression of TGF-β1 could increase the relative expression level of lncRNA4.9 and decrease with time, and reduce the relative expression level of HCMV DNA, thus promoting HCMV into the latent infection state.