Phytochemical analysis of Citrus japonica enrich extract and investigation of Anti-inflammatory activity in Rat Model

Phytochemical analysis of Citrus japonica enrich extract and investigation of Anti-inflammatory activity in Rat Model | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Phytochemical analysis of Citrus japonica enrich extract and investigation of Anti-inflammatory activity in Rat Model zunera Chauhdary, Naheed Akhtar, Maryam Afzaal, Mamoona Tariq, and 2 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-6772850/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract Tangeretin (TAN), a naturally occurring polymethoxylated flavone derived from Citrus japonica peel, exhibited wide biomedical applications. Tangeretin is a hydrophobic compound, it has low solubility and availability, which limits its therapeutic potential. The in vivo anti-inflammatory effect of Tangeretin rich extract (TRE) & Tangeretin loaded nanoparticles (TNPs) (200µg/kg, 400µg/kg, 800µg/kg) were assessed against diclofenac and ibuprofen (10mg/kg) in adult male and female Wister rats using carrageenan and formaldehyde induced inflammation model. The effectiveness of both Tangeretin rich extract and Tangeretin loaded nanoparticles on formaldehyde evoked hallmarks were assessed through biochemical, hematological, radio-graphical and histopathological examination. The ELSA was carried out to assess the expression of interleukin-6 and TNF-α. Level of antioxidant enzymes in liver tissue homogenate were also assessed, the concentration of CRP and RA factor also examined. Furthermore, qPCR was used to access the regulatory effect on inflammatory biomarkers. Tangeretin rich extract and Tangeretin loaded nanoparticles significantly elevate (p <0.001) the level of antioxidant enzyme in contrast to pathological inflammation group. Tangeretin rich extract and Tangeretin-loaded nanoparticles significantly decreased paw swelling and paw diameter (p <.001) in formaldehyde and carrageenan induced inflammation model. Tangeretin rich extract and Tangeretin loaded nanoparticles significantly suppressed the Messenger Ribonucleic Acid expression of pro-inflammatory biomarker also reduced the concentration of antioxidant enzymes in liver tissue homogenate. Therefore, work proved Tangeretin rich extract and Tangeretin loaded nanoparticles possess great potential to prevent and treat inflammatory ailments. Inflammation Tangeretin rich extract Tangeretin loaded nanoparticles Solvent emulsification method Antioxidants Zeta sizer Formaldehyde Carrageenan HPLC Full Text Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-6772850","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":470978562,"identity":"198c1a54-ee09-4478-8040-3675698d9ba1","order_by":0,"name":"zunera 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Antioxidants, Zeta sizer, Formaldehyde, Carrageenan, HPLC","lastPublishedDoi":"10.21203/rs.3.rs-6772850/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-6772850/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"Tangeretin (TAN), a naturally occurring polymethoxylated flavone derived from Citrus japonica peel, exhibited wide biomedical applications. Tangeretin is a hydrophobic compound, it has low solubility and availability, which limits its therapeutic potential. The in vivo anti-inflammatory effect of Tangeretin rich extract (TRE) \u0026amp; Tangeretin loaded nanoparticles (TNPs) (200µg/kg, 400µg/kg, 800µg/kg) were assessed against diclofenac and ibuprofen (10mg/kg) in adult male and female Wister rats using carrageenan and formaldehyde induced inflammation model. The effectiveness of both Tangeretin rich extract and Tangeretin loaded nanoparticles on formaldehyde evoked hallmarks were assessed through biochemical, hematological, radio-graphical and histopathological examination. The ELSA was carried out to assess the expression of interleukin-6 and TNF-α. Level of antioxidant enzymes in liver tissue homogenate were also assessed, the concentration of CRP and RA factor also examined. Furthermore, qPCR was used to access the regulatory effect on inflammatory biomarkers. Tangeretin rich extract and Tangeretin loaded nanoparticles significantly elevate (p \u0026lt;0.001) the level of antioxidant enzyme in contrast to pathological inflammation group. Tangeretin rich extract and Tangeretin-loaded nanoparticles significantly decreased paw swelling and paw diameter (p \u0026lt;.001) in formaldehyde and carrageenan induced inflammation model. Tangeretin rich extract and Tangeretin loaded nanoparticles significantly suppressed the Messenger Ribonucleic Acid expression of pro-inflammatory biomarker also reduced the concentration of antioxidant enzymes in liver tissue homogenate. Therefore, work proved Tangeretin rich extract and Tangeretin loaded nanoparticles possess great potential to prevent and treat inflammatory ailments.","manuscriptTitle":"Phytochemical analysis of Citrus japonica enrich extract and investigation of Anti-inflammatory activity in Rat Model","msid":"","msnumber":"","nonDraftVersions":[{"code":1,"date":"2025-06-17 07:07:08","doi":"10.21203/rs.3.rs-6772850/v1","editorialEvents":[{"type":"communityComments","content":0}],"status":"published","journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true}}],"origin":"","ownerIdentity":"7cd13455-bc39-4f7d-9fe3-c699001cbbb4","owner":[],"postedDate":"June 17th, 2025","published":true,"recentEditorialEvents":[],"rejectedJournal":[],"revision":"","amendment":"","status":"posted","subjectAreas":[],"tags":[],"updatedAt":"2025-07-30T12:20:33+00:00","versionOfRecord":[],"versionCreatedAt":"2025-06-17 07:07:08","video":"","vorDoi":"","vorDoiUrl":"","workflowStages":[]},"version":"v1","identity":"rs-6772850","journalConfig":"researchsquare"},"__N_SSP":true},"page":"/article/[identity]/[[...version]]","query":{"redirect":"/article/rs-6772850","identity":"rs-6772850","version":["v1"]},"buildId":"8U1c8b4HqxoKbykW_rLl7","isFallback":false,"isExperimentalCompile":false,"dynamicIds":[84888],"gssp":true,"scriptLoader":[]}