WITHDRAWN: A highly screening system at seedling-stage for genic male sterility in pepper hybrid breeding

doi:10.21203/rs.3.rs-6529728/v1

WITHDRAWN: A highly screening system at seedling-stage for genic male sterility in pepper hybrid breeding

2025 · doi:10.21203/rs.3.rs-6529728/v1

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Abstract

Abstract In pepper ( Capsicum annuum L.), utilizing heterosis is a highly effective strategy to boost yield. While GMS lines offer advantages for seed production by avoiding the limitations associated with cytoplasmic male sterile (CMS) lines, the use of GMS typically requires the labor-intensive removal of 50% of fertile plants during seed production. To address this challenge, we identified a anthocyanidin-absent (aa) mutant, with a green hypocotyl at seedling stage and yellow anthers.We further identified dihydroflavonol 4-reductase gene ( CaDFR) as a key gene regulating anthocyanin biosynthesis in pepper based on gene fine localization and VIGS experiments. Genetic segregation analysis revealed that CaDFR and CaDYT1 (a genic male sterile gene) had a closely linkage relationship. Building on this discovery, we developed an efficient hybrid seed production strategy using the green hypocotyl morphological marker from the aa mutant and the male sterile line gms1 ( CaDYT1 locus). In conclusion, we successfully cloned CaDFR , a key gene controlling hypocotyl and anther color in pepper. Additionally, we further proposed an efficient seed propagation strategy to accelerate hybrid seed production in the utilization of heterosis. This study not only deepens mechanistic insights into the genetic regulation of pepper pigmentation, but also establishes a practical framework for optimizing hybrid breeding protocols, thereby contributing to simplifying the pepper breeding process and sustainable cultivation.

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WITHDRAWN: A highly screening system at seedling-stage for genic male sterility in pepper hybrid breeding | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article WITHDRAWN: A highly screening system at seedling-stage for genic male sterility in pepper hybrid breeding Pan luzhao, Jin Wang, Peng Tang, Yanlong Li, Yin Luo, Wu Miao, and 11 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-6529728/v2 This work is licensed under a CC BY 4.0 License Status: Posted Version 2 posted You are reading this latest preprint version Show more versions Editorial Note The full text of this preprint has been withdrawn by the authors due to author disagreement with the posting of the preprint. Therefore, the authors do not wish this work to be cited as a reference. Questions should be directed to the corresponding author. Editorial notes are used to provide important context regarding the topic of a preprint or to alert readers to potential issues concerning that preprint or a downstream publication associated with it. For more information on editorial notes, see our Editorial Policies . Abstract The full text of this preprint has been withdrawn by the authors due to author disagreement with the posting of the preprint. Therefore, the authors do not wish this work to be cited as a reference. Questions should be directed to the corresponding author. Full Text The authors have withdrawn this preprint from Research Square. Cite Share Download PDF Status: Posted Version 2 posted You are reading this latest preprint version Show more versions Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. 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