Effect of mixed fermentation of Saccharomyces cerevisiae, Lactobacillus helveticus, Lactobacillus delbrueckii subsp. bulgaricus on physicochemical and flavor characteristics of Pumpkin juice

Effect of mixed fermentation of Saccharomyces cerevisiae, Lactobacillus helveticus, Lactobacillus delbrueckii subsp. bulgaricus on physicochemical and flavor characteristics of Pumpkin juice | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Article Effect of mixed fermentation of Saccharomyces cerevisiae, Lactobacillus helveticus, Lactobacillus delbrueckii subsp. bulgaricus on physicochemical and flavor characteristics of Pumpkin juice Renqin Tan, Chunli Zhou, Manjun Zhang, Wei Su, Shaofang Liu, Leyi Yao, and 2 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-7626401/v1 This work is licensed under a CC BY 4.0 License Status: Published Journal Publication published 28 Nov, 2025 Read the published version in Scientific Reports → Version 1 posted 12 You are reading this latest preprint version Abstract To develop a nutritious and aromatic probiotic-fermented pumpkin juice, fresh pumpkin juice was utilized as the raw material. This juice was co-fermented using Saccharomyces cerevisiae, Lactobacillus helveticus, and Lactobacillus delbrueckii subsp. bulgaricus. Comparative analyses were conducted on the physicochemical properties and aroma components of the fermented pumpkin juice. The results indicate that the viable bacterial count in the fermented pumpkin juice reached 7.34 log CFU mL − 1 . Compared with fresh pumpkin juice, fermented pumpkin juice exhibited significant increases ( P < 0.05) in total acid content, vitamin C, polyphenols, flavonoids, and carotenoids. Additionally, there were significant decreases ( P < 0.05) in pH and soluble solids. The fermentation process was effective in preserving the original color of the pumpkin juice (ΔE < 2). Furthermore, the rheological properties of the pumpkin juice were altered by the composite probiotic fermentation. Notably, GC-MS analysis identified a total of 35 aroma compounds in the pumpkin juice samples, including 9 esters, 8 aldehydes, 5 ketones, 5 acids, 5 alcohols and 3 other substances. The types of esters and alcohols were higher in fermented pumpkin juice compared to fresh pumpkin juice. After OPLS-DA analysis, Prominent aroma compounds detected in the fermented pumpkin juice included phenylethyl alcohol, phenethyl acetate, and ethyl caprylate, which imparted a more intense floral and fruity aroma to the fermented pumpkin juice. And, the pumpkin juice fermented with the composite probiotics received the highest sensory evaluation. Biological sciences/Biochemistry Biological sciences/Biotechnology Biological sciences/Microbiology Mixed fermentation Pumpkin juice Physicochemical property Flavor Full Text Additional Declarations No competing interests reported. Cite Share Download PDF Status: Published Journal Publication published 28 Nov, 2025 Read the published version in Scientific Reports → Version 1 posted Editorial decision: Revision requested 30 Sep, 2025 Reviewers agreed at journal 27 Sep, 2025 Reviews received at journal 26 Sep, 2025 Reviewers agreed at journal 26 Sep, 2025 Reviews received at journal 25 Sep, 2025 Reviewers agreed at journal 25 Sep, 2025 Reviewers agreed at journal 24 Sep, 2025 Reviewers invited by journal 24 Sep, 2025 Editor assigned by journal 24 Sep, 2025 Editor invited by journal 24 Sep, 2025 Submission checks completed at journal 20 Sep, 2025 First submitted to journal 20 Sep, 2025 You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-7626401","acceptedTermsAndConditions":true,"allowDirectSubmit":false,"archivedVersions":[],"articleType":"Article","associatedPublications":[],"authors":[{"id":522915458,"identity":"3b8d184a-952b-49b1-aaae-b7756d675cc8","order_by":0,"name":"Renqin Tan","email":"","orcid":"","institution":"Jiangxi Science \u0026Technology Normal University","correspondingAuthor":false,"prefix":"","firstName":"Renqin","middleName":"","lastName":"Tan","suffix":""},{"id":522915459,"identity":"390f83c8-97b6-46d5-bd28-9ab44e30abf3","order_by":1,"name":"Chunli Zhou","email":"data:image/png;base64,iVBORw0KGgoAAAANSUhEUgAAAZAAAAAyAQMAAABI0h/eAAAABlBMVEX///8AAABVwtN+AAAACXBIWXMAAA7EAAAOxAGVKw4bAAAA1ElEQVRIiWNgGAWjYDACCRBRYCPHxt584MCHCqK1GKQZ8/EcSzw44wzxWg4lzpPIMT7M20KEDvnZzcckfhgcYGxjyPlwgLeBQZ5f7AB+LYxzjqVJ9hjcYWZjOLvhgOQOBsOZsxPwa2GWyDGTZjB4xsbG2LvhgOEZhgSD2wS0sEG0HOZhY+Z5cCCxjQgtPFAtEmxsPAwHDhKjRUIiLdmyxyDNgI2HzeBgwxkJwn6Rn5F88MaPCpv6+fMfP/78p8JGnl+agBYMW0lTPgpGwSgYBaMAOwAAow1CFrVcVmkAAAAASUVORK5CYII=","orcid":"","institution":"Jiangxi Science \u0026Technology Normal University","correspondingAuthor":true,"prefix":"","firstName":"Chunli","middleName":"","lastName":"Zhou","suffix":""},{"id":522915460,"identity":"23ee7c6a-1d63-4c55-90c1-116bbb089c60","order_by":2,"name":"Manjun Zhang","email":"","orcid":"","institution":"Jiangxi Science \u0026Technology Normal University","correspondingAuthor":false,"prefix":"","firstName":"Manjun","middleName":"","lastName":"Zhang","suffix":""},{"id":522915461,"identity":"31a0865f-11ea-4acc-af16-772054545ea4","order_by":3,"name":"Wei Su","email":"","orcid":"","institution":"Jiangxi Science \u0026Technology Normal University","correspondingAuthor":false,"prefix":"","firstName":"Wei","middleName":"","lastName":"Su","suffix":""},{"id":522915462,"identity":"2114d715-bc72-4e7a-acec-4f48e0a2dd1d","order_by":4,"name":"Shaofang Liu","email":"","orcid":"","institution":"Jiangxi Science \u0026Technology Normal University","correspondingAuthor":false,"prefix":"","firstName":"Shaofang","middleName":"","lastName":"Liu","suffix":""},{"id":522915463,"identity":"cab7a867-f1cd-4e18-8c8f-03f2923cd3e1","order_by":5,"name":"Leyi Yao","email":"","orcid":"","institution":"Jiangxi Science \u0026Technology Normal University","correspondingAuthor":false,"prefix":"","firstName":"Leyi","middleName":"","lastName":"Yao","suffix":""},{"id":522915464,"identity":"75ae5d62-7625-47c5-9a99-83e1f0bfebfc","order_by":6,"name":"Wenhui Pan","email":"","orcid":"","institution":"Jiangxi Science \u0026Technology Normal University","correspondingAuthor":false,"prefix":"","firstName":"Wenhui","middleName":"","lastName":"Pan","suffix":""},{"id":522915465,"identity":"ddca45fb-cd40-459b-a4d5-da79cdbbab79","order_by":7,"name":"Wen Li","email":"","orcid":"","institution":"Jiangxi Science \u0026Technology Normal University","correspondingAuthor":false,"prefix":"","firstName":"Wen","middleName":"","lastName":"Li","suffix":""}],"badges":[],"createdAt":"2025-09-16 06:08:28","currentVersionCode":1,"declarations":"","doi":"10.21203/rs.3.rs-7626401/v1","doiUrl":"https://doi.org/10.21203/rs.3.rs-7626401/v1","draftVersion":[],"editorialEvents":[{"content":"https://doi.org/10.1038/s41598-025-26842-z","type":"published","date":"2025-11-28T15:57:19+00:00"}],"editorialNote":"","failedWorkflow":false,"files":[{"id":92983103,"identity":"c3e3a8c3-997c-4a2b-b0f9-4612a839ff8c","added_by":"auto","created_at":"2025-10-07 20:25:17","extension":"docx","order_by":0,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":13234498,"visible":true,"origin":"","legend":"","description":"","filename":"manuscript1.docx","url":"https://assets-eu.researchsquare.com/files/rs-7626401/v1/1da0f0b6cc6e8d4cbf821c40.docx"},{"id":92983105,"identity":"d3ce8344-a867-4b95-bdf8-1c624edb4c8e","added_by":"auto","created_at":"2025-10-07 20:25:17","extension":"json","order_by":1,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":8833,"visible":true,"origin":"","legend":"","description":"","filename":"8dc7509a164046408ae04fd4f8647b49.json","url":"https://assets-eu.researchsquare.com/files/rs-7626401/v1/948b2e3a0e4c9662e6132750.json"},{"id":92983158,"identity":"96b9fc79-db62-43ef-8d81-58511b1ec8cd","added_by":"auto","created_at":"2025-10-07 20:25:21","extension":"xml","order_by":2,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":262848,"visible":true,"origin":"","legend":"","description":"","filename":"8dc7509a164046408ae04fd4f8647b491enriched.xml","url":"https://assets-eu.researchsquare.com/files/rs-7626401/v1/4917d1fcf4ec6159c228a18e.xml"},{"id":92983107,"identity":"ff023159-38f0-42af-a45a-5b1cdf672b74","added_by":"auto","created_at":"2025-10-07 20:25:17","extension":"eps","order_by":3,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":2530,"visible":true,"origin":"","legend":"","description":"","filename":"drawingimage1.eps","url":"https://assets-eu.researchsquare.com/files/rs-7626401/v1/abbf7d8d01d47cb7ee79ca22.eps"},{"id":92983113,"identity":"e8f91ff3-34a2-4744-b33b-18252662a7c1","added_by":"auto","created_at":"2025-10-07 20:25:18","extension":"eps","order_by":4,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":479,"visible":true,"origin":"","legend":"","description":"","filename":"drawingimage2.eps","url":"https://assets-eu.researchsquare.com/files/rs-7626401/v1/60435bb1fe506835f354e570.eps"},{"id":92983133,"identity":"cb13f307-9976-4fbe-9629-357030ffc21b","added_by":"auto","created_at":"2025-10-07 20:25:19","extension":"wmf","order_by":5,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":994,"visible":true,"origin":"","legend":"","description":"","filename":"floatimage1.wmf","url":"https://assets-eu.researchsquare.com/files/rs-7626401/v1/b6c563e0243ac44ffe23e6f6.wmf"},{"id":92983295,"identity":"ee56b4cd-3ccb-4b25-81ae-b4d416b6fb83","added_by":"auto","created_at":"2025-10-07 20:33:21","extension":"jpeg","order_by":6,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":114628,"visible":true,"origin":"","legend":"","description":"","filename":"floatimage10.jpeg","url":"https://assets-eu.researchsquare.com/files/rs-7626401/v1/f0d26407afee4c3dcfc23a25.jpeg"},{"id":92983106,"identity":"816718b5-11d5-4ca3-a710-3a8287075843","added_by":"auto","created_at":"2025-10-07 20:25:17","extension":"jpeg","order_by":7,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":116165,"visible":true,"origin":"","legend":"","description":"","filename":"floatimage11.jpeg","url":"https://assets-eu.researchsquare.com/files/rs-7626401/v1/116fef91a883708b4c4b4268.jpeg"},{"id":92983120,"identity":"735bf9e9-484d-49f7-88ff-b2c79fc9c79f","added_by":"auto","created_at":"2025-10-07 20:25:18","extension":"jpeg","order_by":8,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":533460,"visible":true,"origin":"","legend":"","description":"","filename":"floatimage12.jpeg","url":"https://assets-eu.researchsquare.com/files/rs-7626401/v1/d4bd5f776766302a24679bce.jpeg"},{"id":92983104,"identity":"70220db1-28e5-437f-97d2-047d28457616","added_by":"auto","created_at":"2025-10-07 20:25:17","extension":"png","order_by":9,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":104306,"visible":true,"origin":"","legend":"","description":"","filename":"floatimage13.png","url":"https://assets-eu.researchsquare.com/files/rs-7626401/v1/f1b5f7364ea8beed8a062d40.png"},{"id":92983110,"identity":"0441aa65-f5be-472d-91cd-5cac1f4ca976","added_by":"auto","created_at":"2025-10-07 20:25:17","extension":"jpeg","order_by":10,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":4820600,"visible":true,"origin":"","legend":"","description":"","filename":"floatimage14.jpeg","url":"https://assets-eu.researchsquare.com/files/rs-7626401/v1/2be93a60c3998f49d891d804.jpeg"},{"id":92983149,"identity":"ce3824ff-81d6-4bf4-8692-e8635a9cb601","added_by":"auto","created_at":"2025-10-07 20:25:20","extension":"jpeg","order_by":11,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":2407139,"visible":true,"origin":"","legend":"","description":"","filename":"floatimage15.jpeg","url":"https://assets-eu.researchsquare.com/files/rs-7626401/v1/feb9b95224fb83fe88b1f65d.jpeg"},{"id":92983278,"identity":"a3a6809d-24df-4758-ade5-53c78b0cc068","added_by":"auto","created_at":"2025-10-07 20:33:20","extension":"png","order_by":12,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":262855,"visible":true,"origin":"","legend":"","description":"","filename":"floatimage16.png","url":"https://assets-eu.researchsquare.com/files/rs-7626401/v1/13ab97d3acccd7b69496e7f9.png"},{"id":92983119,"identity":"28b6314f-d22b-4b65-b505-b5631739671d","added_by":"auto","created_at":"2025-10-07 20:25:18","extension":"png","order_by":13,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":263084,"visible":true,"origin":"","legend":"","description":"","filename":"floatimage17.png","url":"https://assets-eu.researchsquare.com/files/rs-7626401/v1/3a0a82368e2f768e32693e22.png"},{"id":92983109,"identity":"ec3513bc-112f-4e57-99b4-bbb414f36228","added_by":"auto","created_at":"2025-10-07 20:25:17","extension":"png","order_by":14,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":320157,"visible":true,"origin":"","legend":"","description":"","filename":"floatimage18.png","url":"https://assets-eu.researchsquare.com/files/rs-7626401/v1/90f87cf46afbd924e1c14361.png"},{"id":92983146,"identity":"d2d8bbc2-4db3-4858-a4b4-9cebd86bdc28","added_by":"auto","created_at":"2025-10-07 20:25:20","extension":"png","order_by":15,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":706414,"visible":true,"origin":"","legend":"","description":"","filename":"floatimage19.png","url":"https://assets-eu.researchsquare.com/files/rs-7626401/v1/e06a7188c3279bf992d5de4c.png"},{"id":92983282,"identity":"c892196a-8ef8-4cca-8cb4-808971193bfc","added_by":"auto","created_at":"2025-10-07 20:33:21","extension":"wmf","order_by":16,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":822,"visible":true,"origin":"","legend":"","description":"","filename":"floatimage2.wmf","url":"https://assets-eu.researchsquare.com/files/rs-7626401/v1/e1c861828c78850e5d8ca166.wmf"},{"id":92983608,"identity":"019a0b50-3bd0-4135-be5d-889688ceef6e","added_by":"auto","created_at":"2025-10-07 20:41:21","extension":"png","order_by":17,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":907820,"visible":true,"origin":"","legend":"","description":"","filename":"floatimage20.png","url":"https://assets-eu.researchsquare.com/files/rs-7626401/v1/08e23adfff65c458f6137dd7.png"},{"id":92983164,"identity":"38c86b17-f80c-42e2-8d91-7091de930f43","added_by":"auto","created_at":"2025-10-07 20:25:22","extension":"png","order_by":18,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":566908,"visible":true,"origin":"","legend":"","description":"","filename":"floatimage21.png","url":"https://assets-eu.researchsquare.com/files/rs-7626401/v1/5637dc01fb8f3d88477e320a.png"},{"id":92983297,"identity":"075ecd80-158f-4141-9a28-ab9a1eee4e7b","added_by":"auto","created_at":"2025-10-07 20:33:22","extension":"png","order_by":19,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":338084,"visible":true,"origin":"","legend":"","description":"","filename":"floatimage22.png","url":"https://assets-eu.researchsquare.com/files/rs-7626401/v1/76f52c07e13fceba85037622.png"},{"id":92983108,"identity":"4020351e-a214-4e32-9f1a-84694601e21e","added_by":"auto","created_at":"2025-10-07 20:25:17","extension":"png","order_by":20,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":270932,"visible":true,"origin":"","legend":"","description":"","filename":"floatimage23.png","url":"https://assets-eu.researchsquare.com/files/rs-7626401/v1/8323a0b8048d5efc384e35d3.png"},{"id":92983114,"identity":"dad0de37-da71-438e-b448-592661d8a3db","added_by":"auto","created_at":"2025-10-07 20:25:18","extension":"png","order_by":21,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":522642,"visible":true,"origin":"","legend":"","description":"","filename":"floatimage24.png","url":"https://assets-eu.researchsquare.com/files/rs-7626401/v1/c3c89d3889d9f3abe101c0de.png"},{"id":92983272,"identity":"89a0dfc4-2950-4a06-9ca8-4f5d0d4242c6","added_by":"auto","created_at":"2025-10-07 20:33:19","extension":"jpeg","order_by":22,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":3683038,"visible":true,"origin":"","legend":"","description":"","filename":"floatimage25.jpeg","url":"https://assets-eu.researchsquare.com/files/rs-7626401/v1/62441a2617d4362669864f5d.jpeg"},{"id":92983139,"identity":"122f40d0-153d-4c11-ac4c-2a2cdf9aec89","added_by":"auto","created_at":"2025-10-07 20:25:20","extension":"wmf","order_by":23,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":1230,"visible":true,"origin":"","legend":"","description":"","filename":"floatimage3.wmf","url":"https://assets-eu.researchsquare.com/files/rs-7626401/v1/dcf2b84da698522225b56337.wmf"},{"id":92983144,"identity":"9a306191-a4ce-4e80-99b0-0c1e6b8342a5","added_by":"auto","created_at":"2025-10-07 20:25:20","extension":"jpeg","order_by":24,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":253607,"visible":true,"origin":"","legend":"","description":"","filename":"floatimage4.jpeg","url":"https://assets-eu.researchsquare.com/files/rs-7626401/v1/53b5a74d50e87174e6250f07.jpeg"},{"id":92983163,"identity":"61d86a46-3852-44ab-91ab-d8ee094d771e","added_by":"auto","created_at":"2025-10-07 20:25:21","extension":"jpeg","order_by":25,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":177589,"visible":true,"origin":"","legend":"","description":"","filename":"floatimage5.jpeg","url":"https://assets-eu.researchsquare.com/files/rs-7626401/v1/231203aa7ea2d17787ba38dc.jpeg"},{"id":92983117,"identity":"a8744055-d9bb-4223-8c68-2b557be2b7d0","added_by":"auto","created_at":"2025-10-07 20:25:18","extension":"jpeg","order_by":26,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":231510,"visible":true,"origin":"","legend":"","description":"","filename":"floatimage6.jpeg","url":"https://assets-eu.researchsquare.com/files/rs-7626401/v1/6a242ca1a81593fcfc9e9c82.jpeg"},{"id":92983276,"identity":"052bb344-ebf0-4d8e-baa6-748058e4cf6f","added_by":"auto","created_at":"2025-10-07 20:33:19","extension":"jpeg","order_by":27,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":171742,"visible":true,"origin":"","legend":"","description":"","filename":"floatimage7.jpeg","url":"https://assets-eu.researchsquare.com/files/rs-7626401/v1/4d0292212fc9e1b2687dfc59.jpeg"},{"id":92983115,"identity":"ab580002-2194-4128-9c38-17ef67b99ece","added_by":"auto","created_at":"2025-10-07 20:25:18","extension":"jpeg","order_by":28,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":109885,"visible":true,"origin":"","legend":"","description":"","filename":"floatimage8.jpeg","url":"https://assets-eu.researchsquare.com/files/rs-7626401/v1/ba51c39406d22d803c07367c.jpeg"},{"id":92983270,"identity":"96b4de26-f77a-45ab-82de-7aaefa559d97","added_by":"auto","created_at":"2025-10-07 20:33:18","extension":"jpeg","order_by":29,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":122280,"visible":true,"origin":"","legend":"","description":"","filename":"floatimage9.jpeg","url":"https://assets-eu.researchsquare.com/files/rs-7626401/v1/e9fe56d889450eb5f9189063.jpeg"},{"id":92983159,"identity":"634e77df-fc0a-4961-bcdb-0ff3af45c32e","added_by":"auto","created_at":"2025-10-07 20:25:21","extension":"wmf","order_by":30,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":994,"visible":true,"origin":"","legend":"","description":"","filename":"floatimage1.wmf","url":"https://assets-eu.researchsquare.com/files/rs-7626401/v1/0e69a0816561102f052631bb.wmf"},{"id":92983155,"identity":"fb377d56-8b56-455b-a72b-8ae1a5730de1","added_by":"auto","created_at":"2025-10-07 20:25:21","extension":"wmf","order_by":31,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":822,"visible":true,"origin":"","legend":"","description":"","filename":"floatimage2.wmf","url":"https://assets-eu.researchsquare.com/files/rs-7626401/v1/2e91491856c2849b31c6ac34.wmf"},{"id":92983151,"identity":"629f7b94-bbaf-4706-948d-4114b57d3a42","added_by":"auto","created_at":"2025-10-07 20:25:21","extension":"wmf","order_by":32,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":1230,"visible":true,"origin":"","legend":"","description":"","filename":"floatimage3.wmf","url":"https://assets-eu.researchsquare.com/files/rs-7626401/v1/efb9989897180f2b1fcdaca8.wmf"},{"id":92983277,"identity":"e4a47146-3734-4ce3-ad43-9ee5ee855066","added_by":"auto","created_at":"2025-10-07 20:33:19","extension":"png","order_by":33,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":713,"visible":true,"origin":"","legend":"","description":"","filename":"Onlinefloatimage1.png","url":"https://assets-eu.researchsquare.com/files/rs-7626401/v1/c28a7dcfa4ef027fb77c5cd3.png"},{"id":92983281,"identity":"6705406c-f131-4cce-ac17-fa56f5a1ba15","added_by":"auto","created_at":"2025-10-07 20:33:20","extension":"png","order_by":34,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":42739,"visible":true,"origin":"","legend":"","description":"","filename":"Onlinefloatimage10.png","url":"https://assets-eu.researchsquare.com/files/rs-7626401/v1/10429235b4e216181d653488.png"},{"id":92983127,"identity":"7ca7e840-c31e-44b6-9c8f-abfda79b0761","added_by":"auto","created_at":"2025-10-07 20:25:19","extension":"png","order_by":35,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":45654,"visible":true,"origin":"","legend":"","description":"","filename":"Onlinefloatimage11.png","url":"https://assets-eu.researchsquare.com/files/rs-7626401/v1/af2d365bcc3fef3d623f1281.png"},{"id":92983143,"identity":"c17949e0-c0a8-4568-8119-e89055382a76","added_by":"auto","created_at":"2025-10-07 20:25:20","extension":"png","order_by":36,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":131733,"visible":true,"origin":"","legend":"","description":"","filename":"Onlinefloatimage12.png","url":"https://assets-eu.researchsquare.com/files/rs-7626401/v1/6cfea3a47ea47d3dc4d991f7.png"},{"id":92983269,"identity":"5691c7be-4c71-46f6-acc4-6049e9ab3e50","added_by":"auto","created_at":"2025-10-07 20:33:17","extension":"png","order_by":37,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":42419,"visible":true,"origin":"","legend":"","description":"","filename":"Onlinefloatimage13.png","url":"https://assets-eu.researchsquare.com/files/rs-7626401/v1/9ba442416960f6176355bc78.png"},{"id":92983168,"identity":"503d6f55-3a34-4586-896c-f45cf3cc1187","added_by":"auto","created_at":"2025-10-07 20:25:22","extension":"png","order_by":38,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":460248,"visible":true,"origin":"","legend":"","description":"","filename":"Onlinefloatimage14.png","url":"https://assets-eu.researchsquare.com/files/rs-7626401/v1/2983d778be660e8c79087c7e.png"},{"id":92983122,"identity":"0338e9bc-4ef5-44db-a9e1-bb334f675911","added_by":"auto","created_at":"2025-10-07 20:25:18","extension":"png","order_by":39,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":381071,"visible":true,"origin":"","legend":"","description":"","filename":"Onlinefloatimage15.png","url":"https://assets-eu.researchsquare.com/files/rs-7626401/v1/7469ab9a8945e00ff10da5ad.png"},{"id":92983294,"identity":"b98e5248-0675-4082-8d04-729a1ed09cbe","added_by":"auto","created_at":"2025-10-07 20:33:21","extension":"png","order_by":40,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":80643,"visible":true,"origin":"","legend":"","description":"","filename":"Onlinefloatimage16.png","url":"https://assets-eu.researchsquare.com/files/rs-7626401/v1/2fdfa9f739166a0f5feb299e.png"},{"id":92983275,"identity":"b17c47b4-b083-4dd6-88e0-88f4f22da6bd","added_by":"auto","created_at":"2025-10-07 20:33:19","extension":"png","order_by":41,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":65602,"visible":true,"origin":"","legend":"","description":"","filename":"Onlinefloatimage17.png","url":"https://assets-eu.researchsquare.com/files/rs-7626401/v1/378f1b583f4f3652911274f7.png"},{"id":92983153,"identity":"b461282f-e9aa-4feb-85e9-dc1a2e236826","added_by":"auto","created_at":"2025-10-07 20:25:21","extension":"png","order_by":42,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":86828,"visible":true,"origin":"","legend":"","description":"","filename":"Onlinefloatimage18.png","url":"https://assets-eu.researchsquare.com/files/rs-7626401/v1/ad5a948b6d087d1472bf03bd.png"},{"id":92983131,"identity":"2cd50e97-6f42-4b73-abd6-a27b82c59882","added_by":"auto","created_at":"2025-10-07 20:25:19","extension":"png","order_by":43,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":273065,"visible":true,"origin":"","legend":"","description":"","filename":"Onlinefloatimage19.png","url":"https://assets-eu.researchsquare.com/files/rs-7626401/v1/78bf676a021afb1bbbc791b1.png"},{"id":92983274,"identity":"f109b24a-bfc3-4934-a1d7-29bcb80a886d","added_by":"auto","created_at":"2025-10-07 20:33:19","extension":"png","order_by":44,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":740,"visible":true,"origin":"","legend":"","description":"","filename":"Onlinefloatimage2.png","url":"https://assets-eu.researchsquare.com/files/rs-7626401/v1/b4a8b7112a9eb6483a10d547.png"},{"id":92983123,"identity":"3b7b4c74-8d95-4873-85ee-064d760d7c4d","added_by":"auto","created_at":"2025-10-07 20:25:18","extension":"png","order_by":45,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":251590,"visible":true,"origin":"","legend":"","description":"","filename":"Onlinefloatimage20.png","url":"https://assets-eu.researchsquare.com/files/rs-7626401/v1/fdf4abfc8e0ec41e08e2faff.png"},{"id":92983280,"identity":"a0941fa0-caaa-4f63-9cfe-0eaa23af2bee","added_by":"auto","created_at":"2025-10-07 20:33:20","extension":"png","order_by":46,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":251342,"visible":true,"origin":"","legend":"","description":"","filename":"Onlinefloatimage21.png","url":"https://assets-eu.researchsquare.com/files/rs-7626401/v1/20256cda493b663ed7a1974a.png"},{"id":92983135,"identity":"e7066d46-9304-4c28-baae-dfc87df49412","added_by":"auto","created_at":"2025-10-07 20:25:19","extension":"png","order_by":47,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":212545,"visible":true,"origin":"","legend":"","description":"","filename":"Onlinefloatimage22.png","url":"https://assets-eu.researchsquare.com/files/rs-7626401/v1/c54eff8b78c965646e85b484.png"},{"id":92983167,"identity":"be9f7933-d4a7-49d6-ba8b-70b7157caf28","added_by":"auto","created_at":"2025-10-07 20:25:22","extension":"png","order_by":48,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":199436,"visible":true,"origin":"","legend":"","description":"","filename":"Onlinefloatimage23.png","url":"https://assets-eu.researchsquare.com/files/rs-7626401/v1/5b15ccd367e570e27792a618.png"},{"id":92983132,"identity":"e37a45d4-6d53-4059-84ec-a37ce1b3ef72","added_by":"auto","created_at":"2025-10-07 20:25:19","extension":"png","order_by":49,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":210704,"visible":true,"origin":"","legend":"","description":"","filename":"Onlinefloatimage24.png","url":"https://assets-eu.researchsquare.com/files/rs-7626401/v1/d4e95281de8b4c625bf40b93.png"},{"id":92983157,"identity":"8d95bc9b-c58c-418b-939b-26ea1df81e31","added_by":"auto","created_at":"2025-10-07 20:25:21","extension":"png","order_by":50,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":644971,"visible":true,"origin":"","legend":"","description":"","filename":"Onlinefloatimage25.png","url":"https://assets-eu.researchsquare.com/files/rs-7626401/v1/bd1665dc7bc7169bce731813.png"},{"id":92983112,"identity":"691a0eb5-3adf-4e49-b2e7-3e490c8f2626","added_by":"auto","created_at":"2025-10-07 20:25:18","extension":"png","order_by":51,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":1048,"visible":true,"origin":"","legend":"","description":"","filename":"Onlinefloatimage3.png","url":"https://assets-eu.researchsquare.com/files/rs-7626401/v1/7d9785b3af919aae34e98679.png"},{"id":92983142,"identity":"02c21242-ccab-4b7e-8246-9aec88f4008f","added_by":"auto","created_at":"2025-10-07 20:25:20","extension":"png","order_by":52,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":632481,"visible":true,"origin":"","legend":"","description":"","filename":"Onlinefloatimage4.png","url":"https://assets-eu.researchsquare.com/files/rs-7626401/v1/38ecfc5b9ef53d4ecf4f61bc.png"},{"id":92983279,"identity":"35bdd2eb-3fe2-4c41-b041-f1132c42171d","added_by":"auto","created_at":"2025-10-07 20:33:20","extension":"png","order_by":53,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":425439,"visible":true,"origin":"","legend":"","description":"","filename":"Onlinefloatimage5.png","url":"https://assets-eu.researchsquare.com/files/rs-7626401/v1/5a1db0f5584728925faefb80.png"},{"id":92983161,"identity":"759cc955-6922-4df7-a4a2-1f91a8b3452f","added_by":"auto","created_at":"2025-10-07 20:25:21","extension":"png","order_by":54,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":546111,"visible":true,"origin":"","legend":"","description":"","filename":"Onlinefloatimage6.png","url":"https://assets-eu.researchsquare.com/files/rs-7626401/v1/ce07236891bf4c868db292ee.png"},{"id":92983296,"identity":"9cf25e25-00e0-43c3-983f-0c7ab3f3fac8","added_by":"auto","created_at":"2025-10-07 20:33:21","extension":"png","order_by":55,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":411217,"visible":true,"origin":"","legend":"","description":"","filename":"Onlinefloatimage7.png","url":"https://assets-eu.researchsquare.com/files/rs-7626401/v1/9caaf12d19d3a7c321b0824d.png"},{"id":92983137,"identity":"b4db5ed1-f8aa-4645-9665-0016662ba6e9","added_by":"auto","created_at":"2025-10-07 20:25:20","extension":"png","order_by":56,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":38423,"visible":true,"origin":"","legend":"","description":"","filename":"Onlinefloatimage8.png","url":"https://assets-eu.researchsquare.com/files/rs-7626401/v1/517a2187ee9b5c74c1efab72.png"},{"id":92983148,"identity":"6fa7c70e-3ae0-42db-ba40-4f3de6c3e2d9","added_by":"auto","created_at":"2025-10-07 20:25:20","extension":"png","order_by":57,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":40480,"visible":true,"origin":"","legend":"","description":"","filename":"Onlinefloatimage9.png","url":"https://assets-eu.researchsquare.com/files/rs-7626401/v1/f050b28198f8470d40d5658f.png"},{"id":92983129,"identity":"341d9ed8-a1e4-45f9-ab57-ad70aa2babd9","added_by":"auto","created_at":"2025-10-07 20:25:19","extension":"png","order_by":58,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":713,"visible":true,"origin":"","legend":"","description":"","filename":"Onlinefloatimage1.png","url":"https://assets-eu.researchsquare.com/files/rs-7626401/v1/ef9a4290722794338275e739.png"},{"id":92983150,"identity":"f49778ea-1aa4-4352-9527-f5a53a7a3d00","added_by":"auto","created_at":"2025-10-07 20:25:20","extension":"png","order_by":59,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":740,"visible":true,"origin":"","legend":"","description":"","filename":"Onlinefloatimage2.png","url":"https://assets-eu.researchsquare.com/files/rs-7626401/v1/149cc4b2ffc978d573ddbac7.png"},{"id":92983271,"identity":"9e0eb7a8-7be6-4945-97ba-81dd32038971","added_by":"auto","created_at":"2025-10-07 20:33:18","extension":"png","order_by":60,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":1048,"visible":true,"origin":"","legend":"","description":"","filename":"Onlinefloatimage3.png","url":"https://assets-eu.researchsquare.com/files/rs-7626401/v1/71f24afb2f6db8d58f6fccf8.png"},{"id":92983141,"identity":"72e31937-886f-42c1-872c-634e6a35208d","added_by":"auto","created_at":"2025-10-07 20:25:20","extension":"xml","order_by":61,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":259643,"visible":true,"origin":"","legend":"","description":"","filename":"8dc7509a164046408ae04fd4f8647b491structuring.xml","url":"https://assets-eu.researchsquare.com/files/rs-7626401/v1/2c7c6f97f2e56748e2f01cf8.xml"},{"id":92983118,"identity":"5a4d272b-9621-42eb-bd5c-32485565f37a","added_by":"auto","created_at":"2025-10-07 20:25:18","extension":"html","order_by":62,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":273863,"visible":true,"origin":"","legend":"","description":"","filename":"earlyproof.html","url":"https://assets-eu.researchsquare.com/files/rs-7626401/v1/2d71692b5674523a01f40c9e.html"},{"id":97178309,"identity":"4d4ffdfb-977c-4a75-a402-a2002790290b","added_by":"auto","created_at":"2025-12-01 16:07:40","extension":"pdf","order_by":1,"title":"","display":"","copyAsset":false,"role":"manuscript-pdf","size":1072529,"visible":true,"origin":"","legend":"","description":"","filename":"manuscript1.pdf","url":"https://assets-eu.researchsquare.com/files/rs-7626401/v1_covered_288d6307-758f-4cfd-8a2b-13261ee46987.pdf"}],"financialInterests":"No competing interests reported.","formattedTitle":"Effect of mixed fermentation of Saccharomyces cerevisiae, Lactobacillus helveticus, Lactobacillus delbrueckii subsp. bulgaricus on physicochemical and flavor characteristics of Pumpkin juice","fulltext":[],"fulltextSource":"","fullText":"","funders":[],"hasAdminPriorityOnWorkflow":false,"hasManuscriptDocX":false,"hasOptedInToPreprint":true,"hasPassedJournalQc":"","hasAnyPriority":false,"hideJournal":false,"highlight":"","institution":"","isAcceptedByJournal":true,"isAuthorSuppliedPdf":true,"isDeskRejected":"","isHiddenFromSearch":false,"isInQc":false,"isInWorkflow":false,"isPdf":true,"isPdfUpToDate":true,"isWithdrawnOrRetracted":false,"journal":{"display":true,"email":"[email protected]","identity":"scientific-reports","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":false,"externalIdentity":"scirep","sideBox":"Learn more about [Scientific Reports](http://www.nature.com/srep/)","snPcode":"","submissionUrl":"","title":"Scientific Reports","twitterHandle":"","acdcEnabled":true,"dfaEnabled":true,"editorialSystem":"stoa","reportingPortfolio":"Scientific Reports","inReviewEnabled":true,"inReviewRevisionsEnabled":true},"keywords":"Mixed fermentation, Pumpkin juice, Physicochemical property, Flavor","lastPublishedDoi":"10.21203/rs.3.rs-7626401/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-7626401/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"\u003cp\u003eTo develop a nutritious and aromatic probiotic-fermented pumpkin juice, fresh pumpkin juice was utilized as the raw material. This juice was co-fermented using \u003cem\u003eSaccharomyces cerevisiae, Lactobacillus helveticus, and Lactobacillus delbrueckii subsp. bulgaricus.\u003c/em\u003e Comparative analyses were conducted on the physicochemical properties and aroma components of the fermented pumpkin juice. The results indicate that the viable bacterial count in the fermented pumpkin juice reached 7.34 log CFU mL\u003csup\u003e\u0026minus;\u0026thinsp;1\u003c/sup\u003e. Compared with fresh pumpkin juice, fermented pumpkin juice exhibited significant increases (\u003cem\u003eP\u003c/em\u003e\u0026thinsp;\u0026lt;\u0026thinsp;0.05) in total acid content, vitamin C, polyphenols, flavonoids, and carotenoids. Additionally, there were significant decreases (\u003cem\u003eP\u003c/em\u003e\u0026thinsp;\u0026lt;\u0026thinsp;0.05) in pH and soluble solids. The fermentation process was effective in preserving the original color of the pumpkin juice (ΔE\u0026thinsp;\u0026lt;\u0026thinsp;2). Furthermore, the rheological properties of the pumpkin juice were altered by the composite probiotic fermentation. Notably, GC-MS analysis identified a total of 35 aroma compounds in the pumpkin juice samples, including 9 esters, 8 aldehydes, 5 ketones, 5 acids, 5 alcohols and 3 other substances. The types of esters and alcohols were higher in fermented pumpkin juice compared to fresh pumpkin juice. After OPLS-DA analysis, Prominent aroma compounds detected in the fermented pumpkin juice included phenylethyl alcohol, phenethyl acetate, and ethyl caprylate, which imparted a more intense floral and fruity aroma to the fermented pumpkin juice. And, the pumpkin juice fermented with the composite probiotics received the highest sensory evaluation.\u003c/p\u003e","manuscriptTitle":"Effect of mixed fermentation of Saccharomyces cerevisiae, Lactobacillus helveticus, Lactobacillus delbrueckii subsp. bulgaricus on physicochemical and flavor characteristics of Pumpkin juice","msid":"","msnumber":"","nonDraftVersions":[{"code":1,"date":"2025-10-07 20:25:11","doi":"10.21203/rs.3.rs-7626401/v1","editorialEvents":[{"type":"communityComments","content":0},{"type":"decision","content":"Revision requested","date":"2025-09-30T13:37:29+00:00","index":"","fulltext":""},{"type":"reviewerAgreed","content":"208133851237733415154123966872344055889","date":"2025-09-27T07:29:49+00:00","index":"hide","fulltext":""},{"type":"editorInvitedReview","content":"","date":"2025-09-26T16:18:41+00:00","index":"hide","fulltext":""},{"type":"reviewerAgreed","content":"254015520643934615108917219731144385355","date":"2025-09-26T16:13:05+00:00","index":"hide","fulltext":""},{"type":"editorInvitedReview","content":"","date":"2025-09-25T13:41:44+00:00","index":"hide","fulltext":""},{"type":"reviewerAgreed","content":"155642472255413644729492231846226642808","date":"2025-09-25T13:10:52+00:00","index":"hide","fulltext":""},{"type":"reviewerAgreed","content":"19122942040706892743769056955277826340","date":"2025-09-24T10:50:51+00:00","index":"hide","fulltext":""},{"type":"reviewersInvited","content":"","date":"2025-09-24T10:39:54+00:00","index":"","fulltext":""},{"type":"editorAssigned","content":"","date":"2025-09-24T10:29:32+00:00","index":"","fulltext":""},{"type":"editorInvited","content":"","date":"2025-09-24T10:10:31+00:00","index":"","fulltext":""},{"type":"checksComplete","content":"","date":"2025-09-20T15:06:59+00:00","index":"","fulltext":""},{"type":"submitted","content":"Scientific Reports","date":"2025-09-20T14:55:39+00:00","index":"","fulltext":""}],"status":"published","journal":{"display":true,"email":"[email protected]","identity":"scientific-reports","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":false,"externalIdentity":"scirep","sideBox":"Learn more about [Scientific Reports](http://www.nature.com/srep/)","snPcode":"","submissionUrl":"","title":"Scientific Reports","twitterHandle":"","acdcEnabled":true,"dfaEnabled":true,"editorialSystem":"stoa","reportingPortfolio":"Scientific Reports","inReviewEnabled":true,"inReviewRevisionsEnabled":true}}],"origin":"","ownerIdentity":"6c198978-849d-49ac-aebc-3726ab939c04","owner":[],"postedDate":"October 7th, 2025","published":true,"recentEditorialEvents":[],"rejectedJournal":[],"revision":"","amendment":"","status":"published-in-journal","subjectAreas":[{"id":55583862,"name":"Biological sciences/Biochemistry"},{"id":55583863,"name":"Biological sciences/Biotechnology"},{"id":55583864,"name":"Biological sciences/Microbiology"}],"tags":[],"updatedAt":"2025-12-01T16:00:39+00:00","versionOfRecord":{"articleIdentity":"rs-7626401","link":"https://doi.org/10.1038/s41598-025-26842-z","journal":{"identity":"scientific-reports","isVorOnly":false,"title":"Scientific Reports"},"publishedOn":"2025-11-28 15:57:19","publishedOnDateReadable":"November 28th, 2025"},"versionCreatedAt":"2025-10-07 20:25:11","video":"","vorDoi":"10.1038/s41598-025-26842-z","vorDoiUrl":"https://doi.org/10.1038/s41598-025-26842-z","workflowStages":[]},"version":"v1","identity":"rs-7626401","journalConfig":"researchsquare"},"__N_SSP":true},"page":"/article/[identity]/[[...version]]","query":{"redirect":"/article/rs-7626401","identity":"rs-7626401","version":["v1"]},"buildId":"8U1c8b4HqxoKbykW_rLl7","isFallback":false,"isExperimentalCompile":false,"dynamicIds":[84888],"gssp":true,"scriptLoader":[]}