Reparative Calcified Barrier Characterization after Mixing Injectable-Platelet Rich Fibrin with Bioactive Direct Pulp Capping Agents; An Exp. Study.

AbstractAim: This study investigated the morphology and localization of hard tissue barriers formed after direct pulp capping using i-PRF mixed with bioactive agents (Mineral Trioxide Aggregate (MTA) and Bioactive Bone Graft (BBG)) in dogs' teeth using Scanning Electron Microscope.Materials and methods:A total of 64 teeth were used out of 8 healthy male mongrel dogs. The teeth were randomly assigned into four groups according to capping agents used, they were exposed and capped as follow, Group A; capped with MTA, Group B; capped with MTA+ i-PRF, Group C; capped with BBG, Group D; capped with BBG + i-PRF. Finally the access cavity was restored with Intermediate Restorative Material (IRM). The dogs were euthanized after 1 month; and samples were then prepared for scanning electron microscopic study. SEM was used to assess the morphology, localization and extension of the reparative hard tissue barriers and using an image-processing and analysis software to delimit the perimeters of the root canal area and the hard tissue barrier to determine the percentage of root canal obliteration. Chi-square test was used for intragroup comparisons. Results showed that all groups were statistically different (P < 0.05), regarding tissue barrier morphology.Conclusion: Addition of i-PRF to pulp capping agents allows production of peripheral hard tissue barriers with more dentinal tubules.