Microwave radiation as an alternative for controlling Colletotrichum in bean seeds

Microwave radiation as an alternative for controlling Colletotrichum in bean seeds | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Microwave radiation as an alternative for controlling Colletotrichum in bean seeds Daniele M. Nascimento, Paula L. Santos, Marcos R. Ribeiro-Junior, and 1 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-8695153/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract Anthracnose, caused by Colletotrichum lindemuthianum (Sacc. & Magnus) Briosi & Cavara, is one of the most damaging diseases affecting common bean (Phaseolus vulgaris L.), often transmitted through infected seeds. While fungicide-based chemical control is widely used, it poses risks such as the emergence of resistant strains and negative environmental impacts. In this context, microwave radiation has emerged as a promising physical method for sustainable seed disinfection. This study aimed to evaluate the effects of microwave radiation on the incidence of C. lindemuthianum and on the physiological quality of artificially inoculated bean seeds. Two frequencies (2450 and 1225 MHz) and three exposure times (30, 45, and 60 seconds) were tested, along with an untreated control. Results showed that longer exposure times, particularly at 2450 MHz, significantly reduced pathogen incidence - most notably in the 60second treatment, which brought infection rates below 2%. However, these same conditions resulted in total seed mortality and compromised germination. In contrast, the treatment at 1225 MHz for 30 seconds achieved a more favorable balance, reducing fungal incidence while maintaining germination rates above 70%. These findings indicate that although microwave radiation is highly effective in suppressing C. lindemuthianum, careful optimization of treatment parameters is essential to preserve seed viability and ensure practical application. seed disinfection seed-borne pathogens physiological viability non-chemical radiation common bean Full Text Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-8695153","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":596375198,"identity":"f9eecc74-8462-47ec-83f7-ad279b80863e","order_by":0,"name":"Daniele M. Nascimento","email":"","orcid":"","institution":"","correspondingAuthor":false,"prefix":"","firstName":"Daniele","middleName":"M.","lastName":"Nascimento","suffix":""},{"id":596375199,"identity":"3fc91be7-ab62-46d4-949c-cdeb88a39bb3","order_by":1,"name":"Paula L. 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While fungicide-based chemical control is widely used, it poses risks such as the emergence of resistant strains and negative environmental impacts. In this context, microwave radiation has emerged as a promising physical method for sustainable seed disinfection. This study aimed to evaluate the effects of microwave radiation on the incidence of C. lindemuthianum and on the physiological quality of artificially inoculated bean seeds. Two frequencies (2450 and 1225 MHz) and three exposure times (30, 45, and 60 seconds) were tested, along with an untreated control. Results showed that longer exposure times, particularly at 2450 MHz, significantly reduced pathogen incidence - most notably in the 60second treatment, which brought infection rates below 2%. However, these same conditions resulted in total seed mortality and compromised germination. In contrast, the treatment at 1225 MHz for 30 seconds achieved a more favorable balance, reducing fungal incidence while maintaining germination rates above 70%. 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