The effect of estrogen drugs on expression of OPN and MMP-9 in endometriosis rats

Objective To investigate the drug(estrogen Alarelin,mifepristone,pregnancy three alkene ketone) on rats with endometriosis EMS) OPN and its effects on MMP-9 expression.Methods The model 36 successful cases of experiments using rats at random divided into four groups,9 cases in each group,were given Alarelin,mifepristone,pregnancy three alkene ketone three kinds of estrogen-like drug intervention,using yeast to intervene,January after rat was killed,its reign and ectopic endometrium out,use the RT-PCR method to the OPN and MMP-9 protein and gene expression analysis,determination.Results The three groups of estrogen drug intervention group in eutopic and ectopic endometrium of OPN and MMP-9 expression were significantly lower than before intervention and after intervention,there was significant difference(P 0.01);the control group after intervention of ectopic endometrium in OPN and the expression of MMP-9 was stronger than the eutopic endometrium;estrogen three group intervention group eutopic and ectopic endometrial expression of OPN there is no statistically significant difference(P 0.05),but after the intervention of ectopic endometrium estrogen intervention group MMP-9 expression was significantly stronger in eutopic endometrium(P 0.01);the control group after intervention of ectopic endometrium in OPNmRNA expression than those before intervention significantly decreased(P 0.01),and reign and ectopic endometrial expression there is no statistically significant difference(P 0.05).Conclusion Estrogen can reduce rat eutopic and ectopic endometrium of OPN and MMP-9 expression ability,and the ability of OPN expression in endometriosis inhibition would be more obvious in the eutopic endometrium,in eutopic endometrium of MMP-9 expression ability of inhibitory role than on heterotopic endometrium;estrogen may pass for local lesions in OPN and MMP-9 expression inhibition was inhibited to reduce cell invasion and adhesion properties.