Characterization of 17β-hydroxysteroid dehydrogenase type 4 in human ovarian surface epithelial cells

Yasuhiro Nagayoshi, Takeshi Ohba, Hiroko Yamamoto, Yuki Miyahara, Hironori Tashiro, Hidetaka Katabuchi, Hitoshi Okamura
In: MHR: Basic science of reproductive medicine · 2005 · vol. 11(9) , pp. 615–621 · doi:10.1093/molehr/gah215 · PMID:16219629 · W2141150967
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Human ovarian surface epithelial cells express 17beta-HSD4 mRNA and protein, suggesting its role in estrogen inactivation and protection against E2 accumulation.

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Abstract

The human ovarian surface epithelium (hOSE) is a single layer of mesothelial-type primitive epithelial cells that are potential estrogen targets. It has been reported that hOSE cells can produce estrogen. However, the mechanisms that regulate estrogen level(s) in hOSE cells are not yet known. To elucidate the enzymes involved in these reactions, we examined gene expression of 17beta-hydroxysteroid dehydrogenases (17beta-HSDs) in primary hOSE (POSE) and OSE2a cells using RT-PCR. We found that POSE cells and cells of the immortalized hOSE line, OSE2a, bidirectionally converted estrone (E1) and 17beta-estradiol (E2). Both cell types expressed mRNA for 17beta-HSD type 1 (17beta-HSD1), suggesting that the enzyme is involved in the E1 to E2 conversion. Interestingly, both cells expressed 17beta-HSD4 mRNA but not 17beta-HSD2 mRNA. We prepared an antibody against the carboxyl terminal of 17beta-HSD4 (anti-17beta-HSD4 antibody), which recognized the 80 and 48 kDa proteins in POSE and OSE2a cells based on immunoblot analysis. Furthermore, immunohistochemical study revealed the presence of 17beta-HSD4 in hOSE cells in the human ovary. These results suggest that 17beta-HSD4 is involved in estrogen inactivation and may protect against an excessive accumulation of E2 in hOSE cells.

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